The isolation and characterisation of primary human laryngeal epithelial cells

Louisa E N Rees, Sinnappa Gunasekaran, Fabian Sipaul, Martin A. Birchall, Michael Bailey

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have shown that the larynx has a prominent immunological component that varies between individuals, and which is influenced by lifestyle factors implicated in the pathogenesis of the inflammatory and neoplastic diseases of the larynx. In order to explore the mechanisms of such links between laryngeal mucosal immunity and the development of lifestyle-related disease, reliable in vitro models are essential. In this study, we isolated and characterised primary laryngeal epithelial cells from normal individuals and show they can be cultured and manipulated to express MHC class II molecules in vitro.

Original languageEnglish (US)
Pages (from-to)725-730
Number of pages6
JournalMolecular Immunology
Volume43
Issue number6
DOIs
StatePublished - Feb 2006
Externally publishedYes

Keywords

  • Epithelial cells
  • MHC class II
  • Serum-free medium
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

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    Rees, L. E. N., Gunasekaran, S., Sipaul, F., Birchall, M. A., & Bailey, M. (2006). The isolation and characterisation of primary human laryngeal epithelial cells. Molecular Immunology, 43(6), 725-730. https://doi.org/10.1016/j.molimm.2005.03.017