The isolation and characterisation of primary human laryngeal epithelial cells

Louisa E N Rees; Sinnappa Gunasekaran; Fabian Sipaul; Martin A. Birchall; Michael Bailey

doi:10.1016/j.molimm.2005.03.017

The isolation and characterisation of primary human laryngeal epithelial cells

Louisa E N Rees, Sinnappa Gunasekaran, Fabian Sipaul, Martin A. Birchall, Michael Bailey

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We have shown that the larynx has a prominent immunological component that varies between individuals, and which is influenced by lifestyle factors implicated in the pathogenesis of the inflammatory and neoplastic diseases of the larynx. In order to explore the mechanisms of such links between laryngeal mucosal immunity and the development of lifestyle-related disease, reliable in vitro models are essential. In this study, we isolated and characterised primary laryngeal epithelial cells from normal individuals and show they can be cultured and manipulated to express MHC class II molecules in vitro.

Original language	English (US)
Pages (from-to)	725-730
Number of pages	6
Journal	Molecular Immunology
Volume	43
Issue number	6
DOIs	https://doi.org/10.1016/j.molimm.2005.03.017
State	Published - Feb 2006
Externally published	Yes

Keywords

Epithelial cells
MHC class II
Serum-free medium
Transplantation

ASJC Scopus subject areas

Molecular Biology
Immunology

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10.1016/j.molimm.2005.03.017

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AU - Rees, Louisa E N

AU - Gunasekaran, Sinnappa

AU - Sipaul, Fabian

AU - Birchall, Martin A.

AU - Bailey, Michael

PY - 2006/2

Y1 - 2006/2

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AB - We have shown that the larynx has a prominent immunological component that varies between individuals, and which is influenced by lifestyle factors implicated in the pathogenesis of the inflammatory and neoplastic diseases of the larynx. In order to explore the mechanisms of such links between laryngeal mucosal immunity and the development of lifestyle-related disease, reliable in vitro models are essential. In this study, we isolated and characterised primary laryngeal epithelial cells from normal individuals and show they can be cultured and manipulated to express MHC class II molecules in vitro.

KW - Epithelial cells

KW - MHC class II

KW - Serum-free medium

KW - Transplantation

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JO - Immunochemistry

JF - Immunochemistry

SN - 0161-5890

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ER -

The isolation and characterisation of primary human laryngeal epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

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