The involvement of the spleen during chronic phase of Schistosoma mansoni infection in galectin-3 -/- mice

Camila Brand, Felipe L. Oliveira, Christina M. Takiya, Antônio Palumbo, Daniel K. Hsu, Fu-Tong Liu, Radovan Borojevic, Roger Chammas, Márcia C. El-Cheikh

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Schistosoma mansoni synthesizes glycoconjugates which interact with galectin-3, eliciting an intense humoral immune response. Moreover, it was demonstrated that galectin-3 regulates B cell differentiation into plasma cells. Splenomegaly is a hallmark event characterized by polyclonal B cell activation and enhancement of antibody production. Here, we investigated whether galectin-3 interferes with spleen organization and B cell compartment during chronic schistosomiasis, using wild type (WT) and galectin-3 -/ mice. In chronically-infected galectin-3 -/ mice the histological architecture of the spleen, including white and red pulps, was disturbed with heterogeneous lymphoid follicles, an increased number of plasma cells (CD19 -B220 -/lowCD138 +) and a reduced number of macrophages (CD19 -B220 -Mac-1 +CD138 -) and B lymphocytes (CD19 +B220 +/highCD138 -), compared with the WT infected mice. In the absence of galectin-3 there was an increase of annexin-V +PI - cells and a major presence of apoptotic cells in spleen compared with WT infected mice. In spleen of WT infected mice galectin-3 was largely expressed in lymphoid follicles and extrafollicular sites. Thus, we propose that galectin-3 plays a role in splenic architecture, controlling distinct events such as apoptosis, macrophage activity, B cell differentiation and plasmacytogenesis in the course of S. mansoni infection.

Original languageEnglish (US)
Pages (from-to)1109-1120
Number of pages12
JournalHistology and Histopathology
Volume27
Issue number8
StatePublished - Aug 2012

Fingerprint

Galectin 3
Schistosomiasis mansoni
Spleen
B-Lymphocytes
Plasma Cells
Cell Differentiation
Macrophages
Glycoconjugates
Schistosoma mansoni
Annexin A5
Schistosomiasis
Splenomegaly
Humoral Immunity
Antibody Formation
Apoptosis

Keywords

  • B-lymphocyte
  • Galectin-3
  • Macrophage
  • Schistosoma mansoni
  • Spleen

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Brand, C., Oliveira, F. L., Takiya, C. M., Palumbo, A., Hsu, D. K., Liu, F-T., ... El-Cheikh, M. C. (2012). The involvement of the spleen during chronic phase of Schistosoma mansoni infection in galectin-3 -/- mice. Histology and Histopathology, 27(8), 1109-1120.

The involvement of the spleen during chronic phase of Schistosoma mansoni infection in galectin-3 -/- mice. / Brand, Camila; Oliveira, Felipe L.; Takiya, Christina M.; Palumbo, Antônio; Hsu, Daniel K.; Liu, Fu-Tong; Borojevic, Radovan; Chammas, Roger; El-Cheikh, Márcia C.

In: Histology and Histopathology, Vol. 27, No. 8, 08.2012, p. 1109-1120.

Research output: Contribution to journalArticle

Brand, C, Oliveira, FL, Takiya, CM, Palumbo, A, Hsu, DK, Liu, F-T, Borojevic, R, Chammas, R & El-Cheikh, MC 2012, 'The involvement of the spleen during chronic phase of Schistosoma mansoni infection in galectin-3 -/- mice', Histology and Histopathology, vol. 27, no. 8, pp. 1109-1120.
Brand C, Oliveira FL, Takiya CM, Palumbo A, Hsu DK, Liu F-T et al. The involvement of the spleen during chronic phase of Schistosoma mansoni infection in galectin-3 -/- mice. Histology and Histopathology. 2012 Aug;27(8):1109-1120.
Brand, Camila ; Oliveira, Felipe L. ; Takiya, Christina M. ; Palumbo, Antônio ; Hsu, Daniel K. ; Liu, Fu-Tong ; Borojevic, Radovan ; Chammas, Roger ; El-Cheikh, Márcia C. / The involvement of the spleen during chronic phase of Schistosoma mansoni infection in galectin-3 -/- mice. In: Histology and Histopathology. 2012 ; Vol. 27, No. 8. pp. 1109-1120.
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