The involvement of the spleen during chronic phase of Schistosoma mansoni infection in galectin-3 -/- mice

Camila Brand, Felipe L. Oliveira, Christina M. Takiya, Antônio Palumbo, Daniel K. Hsu, Fu-Tong Liu, Radovan Borojevic, Roger Chammas, Márcia C. El-Cheikh

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Schistosoma mansoni synthesizes glycoconjugates which interact with galectin-3, eliciting an intense humoral immune response. Moreover, it was demonstrated that galectin-3 regulates B cell differentiation into plasma cells. Splenomegaly is a hallmark event characterized by polyclonal B cell activation and enhancement of antibody production. Here, we investigated whether galectin-3 interferes with spleen organization and B cell compartment during chronic schistosomiasis, using wild type (WT) and galectin-3 -/ mice. In chronically-infected galectin-3 -/ mice the histological architecture of the spleen, including white and red pulps, was disturbed with heterogeneous lymphoid follicles, an increased number of plasma cells (CD19 -B220 -/lowCD138 +) and a reduced number of macrophages (CD19 -B220 -Mac-1 +CD138 -) and B lymphocytes (CD19 +B220 +/highCD138 -), compared with the WT infected mice. In the absence of galectin-3 there was an increase of annexin-V +PI - cells and a major presence of apoptotic cells in spleen compared with WT infected mice. In spleen of WT infected mice galectin-3 was largely expressed in lymphoid follicles and extrafollicular sites. Thus, we propose that galectin-3 plays a role in splenic architecture, controlling distinct events such as apoptosis, macrophage activity, B cell differentiation and plasmacytogenesis in the course of S. mansoni infection.

Original languageEnglish (US)
Pages (from-to)1109-1120
Number of pages12
JournalHistology and Histopathology
Volume27
Issue number8
StatePublished - Aug 2012

Keywords

  • B-lymphocyte
  • Galectin-3
  • Macrophage
  • Schistosoma mansoni
  • Spleen

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

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