The involvement of protein kinase C in activation-induced cell death in T-cell hybridoma

Lee-Way Jin, Kayo Inaba, Tsunao Saitoh

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(α), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(α) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma.

Original languageEnglish (US)
Pages (from-to)217-227
Number of pages11
JournalCellular Immunology
Volume144
Issue number1
DOIs
StatePublished - Oct 1 1992

Fingerprint

Hybridomas
Protein Kinase C
Cell Death
T-Lymphocytes
Down-Regulation
Phorbol Esters
Surface Antigens
T-Cell Antigen Receptor
Plastics
Anti-Idiotypic Antibodies
Protein Isoforms
Phosphorylation
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

The involvement of protein kinase C in activation-induced cell death in T-cell hybridoma. / Jin, Lee-Way; Inaba, Kayo; Saitoh, Tsunao.

In: Cellular Immunology, Vol. 144, No. 1, 01.10.1992, p. 217-227.

Research output: Contribution to journalArticle

@article{00c96c1318a84aae8ee2c160ac36a164,
title = "The involvement of protein kinase C in activation-induced cell death in T-cell hybridoma",
abstract = "T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(α), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(α) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma.",
author = "Lee-Way Jin and Kayo Inaba and Tsunao Saitoh",
year = "1992",
month = "10",
day = "1",
doi = "10.1016/0008-8749(92)90238-K",
language = "English (US)",
volume = "144",
pages = "217--227",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The involvement of protein kinase C in activation-induced cell death in T-cell hybridoma

AU - Jin, Lee-Way

AU - Inaba, Kayo

AU - Saitoh, Tsunao

PY - 1992/10/1

Y1 - 1992/10/1

N2 - T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(α), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(α) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma.

AB - T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(α), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(α) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma.

UR - http://www.scopus.com/inward/record.url?scp=0026706266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026706266&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(92)90238-K

DO - 10.1016/0008-8749(92)90238-K

M3 - Article

C2 - 1394438

AN - SCOPUS:0026706266

VL - 144

SP - 217

EP - 227

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -