Abstract
T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(α), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(α) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 217-227 |
| Number of pages | 11 |
| Journal | Cellular Immunology |
| Volume | 144 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1 1992 |
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ASJC Scopus subject areas
- Cell Biology
- Immunology
Cite this
The involvement of protein kinase C in activation-induced cell death in T-cell hybridoma. / Jin, Lee-Way; Inaba, Kayo; Saitoh, Tsunao.
In: Cellular Immunology, Vol. 144, No. 1, 01.10.1992, p. 217-227.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The involvement of protein kinase C in activation-induced cell death in T-cell hybridoma
AU - Jin, Lee-Way
AU - Inaba, Kayo
AU - Saitoh, Tsunao
PY - 1992/10/1
Y1 - 1992/10/1
N2 - T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(α), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(α) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma.
AB - T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(α), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(α) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma.
UR - http://www.scopus.com/inward/record.url?scp=0026706266&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026706266&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(92)90238-K
DO - 10.1016/0008-8749(92)90238-K
M3 - Article
C2 - 1394438
AN - SCOPUS:0026706266
VL - 144
SP - 217
EP - 227
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 1
ER -