TY - JOUR
T1 - The involucrin gene of the tree shrew
T2 - Recent repeat additions and the relocation of cysteine codons
AU - Phillips, Marjorie
AU - Rice, Robert H.
AU - Djian, Philippe
AU - Green, Howard
PY - 1997/3/10
Y1 - 1997/3/10
N2 - The coding region of the involucrin gene of Tupaia glis has been cloned and sequenced. It resembles the involucrin coding region of other non-anthropoid mammals in possessing a segment of related, short tandem repeats at a defined location, but in Tupaia, there has been recent serial duplication of a repeat into which a cysteine codon had earlier been introduced. As a result of the duplication, there is a total of as many as six cysteine codons in the segment of repeats, a number larger than for any other species yet examined. In Rattus there has been a comparable but independent addition of cysteine codons, and both Tupaia and Rattus have eliminated an otherwise conserved cysteine codon 75 located close to but outside the segment of repeats. In Tupaia, this elimination probably occurred by gene conversion. Also independently, the gene of Canis has added cysteine codons to the segment of repeats but has not yet lost cysteine 75. It is proposed that the gain and the loss of cysteine codons are parts of a multi-stage program of cysteine relocation.
AB - The coding region of the involucrin gene of Tupaia glis has been cloned and sequenced. It resembles the involucrin coding region of other non-anthropoid mammals in possessing a segment of related, short tandem repeats at a defined location, but in Tupaia, there has been recent serial duplication of a repeat into which a cysteine codon had earlier been introduced. As a result of the duplication, there is a total of as many as six cysteine codons in the segment of repeats, a number larger than for any other species yet examined. In Rattus there has been a comparable but independent addition of cysteine codons, and both Tupaia and Rattus have eliminated an otherwise conserved cysteine codon 75 located close to but outside the segment of repeats. In Tupaia, this elimination probably occurred by gene conversion. Also independently, the gene of Canis has added cysteine codons to the segment of repeats but has not yet lost cysteine 75. It is proposed that the gain and the loss of cysteine codons are parts of a multi-stage program of cysteine relocation.
KW - Gene conversion
KW - Protein evolution
KW - Tandem repeats
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U2 - 10.1016/S0378-1119(96)00654-3
DO - 10.1016/S0378-1119(96)00654-3
M3 - Article
C2 - 9073063
AN - SCOPUS:0031050966
VL - 187
SP - 29
EP - 34
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -