The intrinsic incompressibility of osteoblast-like cells

Robert P. Wilkes, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This paper presents a new methodology, apparatus design, and the experimental results of ongoing research into the measurement of the mechanical properties of musculoskeletal tissue at the cellular level. A microchamber was constructed that provides a controlled hydrostatic pressure environment for these cells where optical sectioning, via epifluorescence microscopy, was used to acquire volume information about the individual cell. The microchamber was integrated into a hydraulic system that, via computer control, provided a regulated adjustable hydrostatic pressure environment for living cells suspended in culture media. The techniques applied in this study include fluorescent labeling of the cell volume, hydrostatic pressure application, optical sectioning, and digital volume reconstruction. To determine the mechanical response (compressibility) of cultured MG-63 osteoblast-like cells under physiologically high hydrostatic pressures two experiments were devised: In the first experiment changes in volume of 10 cells were measured as the applied hydrostatic pressure was increased from 0 to 7 MPa. Volume changes in response to pressure magnitudes were not significant (p > 0.49). In the second experiment, the mechanical role of the plasma membrane to act as a supportive component in cell compressibility was studied by permeabilizing the membrane of six cells and again applying hydrostatic pressure. Again, no significant volume differences between pressurized and unpressurized cells were found (p > 0.46). A retrospective power analysis of the results of the first and second experiments indicates that the sample size was sufficient. The results of this study show that MG- 63 osteoblast-like cells are intrinsically incompressible in the 0-7 MPa hydrostatic pressure range. They also support the hypothesis that the plasma membrane plays an insignificant mechanical role in terms of cell compressibility.

Original languageEnglish (US)
Pages (from-to)167-181
Number of pages15
JournalTissue Engineering
Volume2
Issue number3
StatePublished - Sep 1996
Externally publishedYes

Fingerprint

Hydrostatic Pressure
Osteoblasts
Hydrostatic pressure
Compressibility
Cell Membrane
Cell membranes
Cell Size
Experiments
Computer control
Computer Systems
Cellular Structures
Cell culture
Sample Size
Labeling
Culture Media
Microscopy
Microscopic examination
Research Design
Cells
Hydraulics

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology

Cite this

Wilkes, R. P., & Athanasiou, K. A. (1996). The intrinsic incompressibility of osteoblast-like cells. Tissue Engineering, 2(3), 167-181.

The intrinsic incompressibility of osteoblast-like cells. / Wilkes, Robert P.; Athanasiou, Kyriacos A.

In: Tissue Engineering, Vol. 2, No. 3, 09.1996, p. 167-181.

Research output: Contribution to journalArticle

Wilkes, RP & Athanasiou, KA 1996, 'The intrinsic incompressibility of osteoblast-like cells', Tissue Engineering, vol. 2, no. 3, pp. 167-181.
Wilkes RP, Athanasiou KA. The intrinsic incompressibility of osteoblast-like cells. Tissue Engineering. 1996 Sep;2(3):167-181.
Wilkes, Robert P. ; Athanasiou, Kyriacos A. / The intrinsic incompressibility of osteoblast-like cells. In: Tissue Engineering. 1996 ; Vol. 2, No. 3. pp. 167-181.
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