TY - JOUR
T1 - The internal polyadenylate tract of yeast killer virus M1 double-stranded RNA is variable in length
AU - Hannig, Ernest M.
AU - Williams, Teresa L.
AU - Leibowitz, Michael J
PY - 1986/7/15
Y1 - 1986/7/15
N2 - The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized (+) transcript (denoted m) of M1 dsRNA, neither of which contains 3′-terminal polyadenylate. We now show that there is variability in the length of the polyadenylate tracts of m transcripts synthesized in vitro by virions purified from either of the K1 diploid killer strains A364A × S7 or A364A × 1384. This variability reflects size differences seen in the corresponding M1 dsRNA genomes which, along with other data presented, localizes the variability in the length of M1 dsRNA to the adenine- and uracil-rich region.
AB - The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized (+) transcript (denoted m) of M1 dsRNA, neither of which contains 3′-terminal polyadenylate. We now show that there is variability in the length of the polyadenylate tracts of m transcripts synthesized in vitro by virions purified from either of the K1 diploid killer strains A364A × S7 or A364A × 1384. This variability reflects size differences seen in the corresponding M1 dsRNA genomes which, along with other data presented, localizes the variability in the length of M1 dsRNA to the adenine- and uracil-rich region.
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U2 - 10.1016/0042-6822(86)90380-6
DO - 10.1016/0042-6822(86)90380-6
M3 - Article
C2 - 3521070
AN - SCOPUS:0022496841
VL - 152
SP - 149
EP - 158
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -