Abstract
The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized (+) transcript (denoted m) of M1 dsRNA, neither of which contains 3′-terminal polyadenylate. We now show that there is variability in the length of the polyadenylate tracts of m transcripts synthesized in vitro by virions purified from either of the K1 diploid killer strains A364A × S7 or A364A × 1384. This variability reflects size differences seen in the corresponding M1 dsRNA genomes which, along with other data presented, localizes the variability in the length of M1 dsRNA to the adenine- and uracil-rich region.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 149-158 |
| Number of pages | 10 |
| Journal | Virology |
| Volume | 152 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 15 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
The internal polyadenylate tract of yeast killer virus M1 double-stranded RNA is variable in length. / Hannig, Ernest M.; Williams, Teresa L.; Leibowitz, Michael J.
In: Virology, Vol. 152, No. 1, 15.07.1986, p. 149-158.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The internal polyadenylate tract of yeast killer virus M1 double-stranded RNA is variable in length
AU - Hannig, Ernest M.
AU - Williams, Teresa L.
AU - Leibowitz, Michael J
PY - 1986/7/15
Y1 - 1986/7/15
N2 - The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized (+) transcript (denoted m) of M1 dsRNA, neither of which contains 3′-terminal polyadenylate. We now show that there is variability in the length of the polyadenylate tracts of m transcripts synthesized in vitro by virions purified from either of the K1 diploid killer strains A364A × S7 or A364A × 1384. This variability reflects size differences seen in the corresponding M1 dsRNA genomes which, along with other data presented, localizes the variability in the length of M1 dsRNA to the adenine- and uracil-rich region.
AB - The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized (+) transcript (denoted m) of M1 dsRNA, neither of which contains 3′-terminal polyadenylate. We now show that there is variability in the length of the polyadenylate tracts of m transcripts synthesized in vitro by virions purified from either of the K1 diploid killer strains A364A × S7 or A364A × 1384. This variability reflects size differences seen in the corresponding M1 dsRNA genomes which, along with other data presented, localizes the variability in the length of M1 dsRNA to the adenine- and uracil-rich region.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022496841&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(86)90380-6
DO - 10.1016/0042-6822(86)90380-6
M3 - Article
C2 - 3521070
AN - SCOPUS:0022496841
VL - 152
SP - 149
EP - 158
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -