The initiation of embryonic-like collagen fibrillogenesis by adult human tendon fibroblasts when cultured under tension

Monika L. Bayer, Chin Yan C Yeung, Karl E. Kadler, Klaus Qvortrup, Keith Baar, René B. Svensson, S. Peter Magnusson, Michael Krogsgaard, Manuel Koch, Michael Kjaer

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Tendon fibroblasts synthesize collagen and form fibrils during embryonic development, but to what extent mature fibroblasts are able to recapitulate embryonic development and develop normal tendon structure is unknown. The present study examined the capability of mature human tendon fibroblasts to initiate collagen fibrillogenesis when cultured in fixed-length fibrin gels. Fibroblasts were dissected from semitendinosus and gracilis tendons from healthy humans and cultured in 3D linear fibrin gels. The fibroblasts synthesized an extracellular matrix of parallel collagen fibrils that were aligned along the axis of tension. The fibrils had a homogeneous narrow diameter that was similar to collagen fibrils occurring in embryonic tendon. Immunostaining showed colocalization of collagen type I with collagen III, XII and XIV. A fibronectin network was formed in parallel with the collagen, and fibroblasts stained positive for integrin α5. Finally, the presence of cell extensions into the extracellular space with membrane-enclosed fibrils in fibripositors indicated characteristics of embryonic tendon. We conclude that mature human tendon fibroblasts retain an intrinsic capability to perform collagen fibrillogenesis similar to that of developing tendon, which implies that the hormonal/mechanical milieu, rather than intrinsic cellular function, inhibits regenerative potential in mature tendon.

Original languageEnglish (US)
Pages (from-to)4889-4897
Number of pages9
JournalBiomaterials
Volume31
Issue number18
DOIs
StatePublished - Jun 2010

Fingerprint

Tendons
Fibroblasts
Collagen
Fibrin
Embryonic Development
Gels
Extracellular Space
Collagen Type I
Fibronectins
Integrins
Extracellular Matrix
Membranes

Keywords

  • Collagen
  • Extracellular matrix
  • Fibroblast
  • Tendon

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Bayer, M. L., Yeung, C. Y. C., Kadler, K. E., Qvortrup, K., Baar, K., Svensson, R. B., ... Kjaer, M. (2010). The initiation of embryonic-like collagen fibrillogenesis by adult human tendon fibroblasts when cultured under tension. Biomaterials, 31(18), 4889-4897. https://doi.org/10.1016/j.biomaterials.2010.02.062

The initiation of embryonic-like collagen fibrillogenesis by adult human tendon fibroblasts when cultured under tension. / Bayer, Monika L.; Yeung, Chin Yan C; Kadler, Karl E.; Qvortrup, Klaus; Baar, Keith; Svensson, René B.; Peter Magnusson, S.; Krogsgaard, Michael; Koch, Manuel; Kjaer, Michael.

In: Biomaterials, Vol. 31, No. 18, 06.2010, p. 4889-4897.

Research output: Contribution to journalArticle

Bayer, ML, Yeung, CYC, Kadler, KE, Qvortrup, K, Baar, K, Svensson, RB, Peter Magnusson, S, Krogsgaard, M, Koch, M & Kjaer, M 2010, 'The initiation of embryonic-like collagen fibrillogenesis by adult human tendon fibroblasts when cultured under tension', Biomaterials, vol. 31, no. 18, pp. 4889-4897. https://doi.org/10.1016/j.biomaterials.2010.02.062
Bayer, Monika L. ; Yeung, Chin Yan C ; Kadler, Karl E. ; Qvortrup, Klaus ; Baar, Keith ; Svensson, René B. ; Peter Magnusson, S. ; Krogsgaard, Michael ; Koch, Manuel ; Kjaer, Michael. / The initiation of embryonic-like collagen fibrillogenesis by adult human tendon fibroblasts when cultured under tension. In: Biomaterials. 2010 ; Vol. 31, No. 18. pp. 4889-4897.
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