Abstract
Verticillium wilt of lettuce caused by Verticillium dahliae can cause severe economic damage to lettuce producers. Complete resistance to race 1 isolates is available in Lactuca sativa cultivar (cv.) La Brillante and understanding the genetic basis of this resistance will aid development of new resistant cultivars. F 1 and F 2 families from crosses between La Brillante and three iceberg cultivars as well as a recombinant inbred line population derived from L. sativa cv. Salinas 88 × La Brillante were evaluated for disease incidence and disease severity in replicated greenhouse and field experiments. One hundred and six molecular markers were used to generate a genetic map from Salinas 88 × La Brillante and for detection of quantitative trait loci. Segregation was consistent with a single dominant gene of major effect which we are naming Verticillium resistance 1 (Vr1). The gene described large portions of the phenotypic variance (R 2 = 0.49-0.68) and was mapped to linkage group 9 coincident with an expressed sequence tag marker (QGD8I16. yg. ab1) that has sequence similarity with the Ve gene that confers resistance to V. dahliae race 1 in tomato. The simple inheritance of resistance indicates that breeding procedures designed for single genes will be applicable for developing resistant cultivars. QGD8I16. yg. ab1 is a good candidate for functional analysis and development of markers suitable for marker-assisted selection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 509-517 |
| Number of pages | 9 |
| Journal | Theoretical And Applied Genetics |
| Volume | 123 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 2011 |
Fingerprint
ASJC Scopus subject areas
- Agronomy and Crop Science
- Genetics
- Biotechnology
Cite this
The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. / Hayes, Ryan J.; McHale, Leah K.; Vallad, Gary E.; Truco, Maria Jose; Michelmore, Richard W; Klosterman, Steve J.; Maruthachalam, Karunakaran; Subbarao, Krishna V.
In: Theoretical And Applied Genetics, Vol. 123, No. 4, 08.2011, p. 509-517.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante
AU - Hayes, Ryan J.
AU - McHale, Leah K.
AU - Vallad, Gary E.
AU - Truco, Maria Jose
AU - Michelmore, Richard W
AU - Klosterman, Steve J.
AU - Maruthachalam, Karunakaran
AU - Subbarao, Krishna V.
PY - 2011/8
Y1 - 2011/8
N2 - Verticillium wilt of lettuce caused by Verticillium dahliae can cause severe economic damage to lettuce producers. Complete resistance to race 1 isolates is available in Lactuca sativa cultivar (cv.) La Brillante and understanding the genetic basis of this resistance will aid development of new resistant cultivars. F 1 and F 2 families from crosses between La Brillante and three iceberg cultivars as well as a recombinant inbred line population derived from L. sativa cv. Salinas 88 × La Brillante were evaluated for disease incidence and disease severity in replicated greenhouse and field experiments. One hundred and six molecular markers were used to generate a genetic map from Salinas 88 × La Brillante and for detection of quantitative trait loci. Segregation was consistent with a single dominant gene of major effect which we are naming Verticillium resistance 1 (Vr1). The gene described large portions of the phenotypic variance (R 2 = 0.49-0.68) and was mapped to linkage group 9 coincident with an expressed sequence tag marker (QGD8I16. yg. ab1) that has sequence similarity with the Ve gene that confers resistance to V. dahliae race 1 in tomato. The simple inheritance of resistance indicates that breeding procedures designed for single genes will be applicable for developing resistant cultivars. QGD8I16. yg. ab1 is a good candidate for functional analysis and development of markers suitable for marker-assisted selection.
AB - Verticillium wilt of lettuce caused by Verticillium dahliae can cause severe economic damage to lettuce producers. Complete resistance to race 1 isolates is available in Lactuca sativa cultivar (cv.) La Brillante and understanding the genetic basis of this resistance will aid development of new resistant cultivars. F 1 and F 2 families from crosses between La Brillante and three iceberg cultivars as well as a recombinant inbred line population derived from L. sativa cv. Salinas 88 × La Brillante were evaluated for disease incidence and disease severity in replicated greenhouse and field experiments. One hundred and six molecular markers were used to generate a genetic map from Salinas 88 × La Brillante and for detection of quantitative trait loci. Segregation was consistent with a single dominant gene of major effect which we are naming Verticillium resistance 1 (Vr1). The gene described large portions of the phenotypic variance (R 2 = 0.49-0.68) and was mapped to linkage group 9 coincident with an expressed sequence tag marker (QGD8I16. yg. ab1) that has sequence similarity with the Ve gene that confers resistance to V. dahliae race 1 in tomato. The simple inheritance of resistance indicates that breeding procedures designed for single genes will be applicable for developing resistant cultivars. QGD8I16. yg. ab1 is a good candidate for functional analysis and development of markers suitable for marker-assisted selection.
UR - http://www.scopus.com/inward/record.url?scp=79961166586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79961166586&partnerID=8YFLogxK
U2 - 10.1007/s00122-011-1603-y
DO - 10.1007/s00122-011-1603-y
M3 - Article
C2 - 21567237
AN - SCOPUS:79961166586
VL - 123
SP - 509
EP - 517
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
SN - 0040-5752
IS - 4
ER -