The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante

Ryan J. Hayes, Leah K. McHale, Gary E. Vallad, Maria Jose Truco, Richard W Michelmore, Steve J. Klosterman, Karunakaran Maruthachalam, Krishna V. Subbarao

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Verticillium wilt of lettuce caused by Verticillium dahliae can cause severe economic damage to lettuce producers. Complete resistance to race 1 isolates is available in Lactuca sativa cultivar (cv.) La Brillante and understanding the genetic basis of this resistance will aid development of new resistant cultivars. F 1 and F 2 families from crosses between La Brillante and three iceberg cultivars as well as a recombinant inbred line population derived from L. sativa cv. Salinas 88 × La Brillante were evaluated for disease incidence and disease severity in replicated greenhouse and field experiments. One hundred and six molecular markers were used to generate a genetic map from Salinas 88 × La Brillante and for detection of quantitative trait loci. Segregation was consistent with a single dominant gene of major effect which we are naming Verticillium resistance 1 (Vr1). The gene described large portions of the phenotypic variance (R 2 = 0.49-0.68) and was mapped to linkage group 9 coincident with an expressed sequence tag marker (QGD8I16. yg. ab1) that has sequence similarity with the Ve gene that confers resistance to V. dahliae race 1 in tomato. The simple inheritance of resistance indicates that breeding procedures designed for single genes will be applicable for developing resistant cultivars. QGD8I16. yg. ab1 is a good candidate for functional analysis and development of markers suitable for marker-assisted selection.

Original languageEnglish (US)
Pages (from-to)509-517
Number of pages9
JournalTheoretical And Applied Genetics
Volume123
Issue number4
DOIs
StatePublished - Aug 2011

Fingerprint

Verticillium
Lettuce
Verticillium wilt
Verticillium dahliae
lettuce
inheritance (genetics)
Lactuca sativa
cultivars
Genes
icebergs
Ice Cover
Dominant Genes
development aid
genes
Quantitative Trait Loci
Expressed Sequence Tags
dominant genes
Lycopersicon esculentum
expressed sequence tags
linkage groups

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Biotechnology

Cite this

Hayes, R. J., McHale, L. K., Vallad, G. E., Truco, M. J., Michelmore, R. W., Klosterman, S. J., ... Subbarao, K. V. (2011). The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Theoretical And Applied Genetics, 123(4), 509-517. https://doi.org/10.1007/s00122-011-1603-y

The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. / Hayes, Ryan J.; McHale, Leah K.; Vallad, Gary E.; Truco, Maria Jose; Michelmore, Richard W; Klosterman, Steve J.; Maruthachalam, Karunakaran; Subbarao, Krishna V.

In: Theoretical And Applied Genetics, Vol. 123, No. 4, 08.2011, p. 509-517.

Research output: Contribution to journalArticle

Hayes, RJ, McHale, LK, Vallad, GE, Truco, MJ, Michelmore, RW, Klosterman, SJ, Maruthachalam, K & Subbarao, KV 2011, 'The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante', Theoretical And Applied Genetics, vol. 123, no. 4, pp. 509-517. https://doi.org/10.1007/s00122-011-1603-y
Hayes, Ryan J. ; McHale, Leah K. ; Vallad, Gary E. ; Truco, Maria Jose ; Michelmore, Richard W ; Klosterman, Steve J. ; Maruthachalam, Karunakaran ; Subbarao, Krishna V. / The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. In: Theoretical And Applied Genetics. 2011 ; Vol. 123, No. 4. pp. 509-517.
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