The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving

Peter A. Beal, Peter B. Dervan

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

The influence of sixteen base triplet changes at a single position within a pur·pur·pyr triple helix was examined by affinity cleaving. For the 15 base pair target site studied here, G·GC, A·AT and T·AT triplets stabilize a triple helix to a greater extent than the other 13 natural triplets (pH = 7.4, 25deg;C). Weaker interactions were detected for the C·AT, A·GC and T·CG triplets. The absence of specific, highly stabilizing interactions between third strand bases and the CG or TA base pairs demonstrates a current sequence limitation to formation of this structure. Models for the two dimensional base triplet interactions for all possible 16 natural triplets are presented.

Original languageEnglish (US)
Pages (from-to)2773-2776
Number of pages4
JournalNucleic Acids Research
Volume20
Issue number11
StatePublished - Jun 11 1992
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

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