The influence of manganese supplementation on seizure onset and severity, and brain monoamines in the genetically epilepsy prone rat

James W. Critchfield, G. Frank Carl, Carl L Keen

Research output: Contribution to journalArticle

25 Scopus citations


Human and experimental animal studies suggest a relationship between low Mn status and seizures. The genetically epilepsy prone rat (GEPR), which has low tissue Mn levels, was studied in the context of Mn supplementation. Manganese was provided at 45 μg/g diet (control) or 1000 μg/g diet (supplemented) to dams during pregnancy and lactation, then to the offspring after weaning. Offspring were tested for seizure susceptibility as young adults; tissue trace elements, brain monoamines and brain glutamine synthetase activity were measured as endpoint biochemical indices. Supplementation, although developmentally encompassing and highly effective in elevating tissue Mn levels, had no effect on seizure latency or severity. Similarly, brain monoamine concentrations and glutamine synthetase activities were resistant to Mn supplementation. Notably, the GEPR was confirmed to have low whole brain glutamine synthetase activity. These findings suggest that seizure activity in the GEPR does not stem from an increased nutritional/metabolic need for Mn.

Original languageEnglish (US)
Pages (from-to)3-10
Number of pages8
JournalEpilepsy Research
Issue number1
StatePublished - 1993



  • (Epilepsy)
  • Genetically epilepsy prone rat
  • Glutamine synthetase
  • Manganese
  • Scizures

ASJC Scopus subject areas

  • Clinical Neurology
  • Pediatrics, Perinatology, and Child Health
  • Neurology

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