The influence of electron beam sterilization on in vivo degradation of (β-TCP/PCL of different composite ratios for bone tissue engineering

Jin Ho Kang, Janelle Kaneda, Jae Gon Jang, Kumaresan Sakthiabirami, Elaine Lui, Carolyn Kim, Aijun Wang, Sang Won Park, Yunzhi Peter Yang

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

We evaluated the effect of electron beam (E-beam) sterilization (25 kGy, ISO 11137) on the degradation of 3-tricalcium phosphate/polycaprolactone (β3-TCP/PCL) composite filaments of various ratios (0:100, 20:80, 40:60, and 60:40 TCP:PCL by mass) in a rat subcutaneous model for 24 weeks. Volumes of the samples before implantation and after explantation were measured using micro-computed tomography (micro-CT). The filament volume changes before sacrifice were also measured using a live micro-CT. In our micro-CT analyses, there was no significant difference in volume change between the E-beam treated groups and non-E-beam treated groups of the same 3-TCP to PCL ratios, except for the 0% (β-TCP group. However, the average volume reduction differences between the E-beam and non-E-beam groups in the same-ratio samples were 0.76% (0% TCP), 3.30% (20% TCP), 4.65% (40% TCP), and 3.67% (60% TCP). The E-beam samples generally had more volume reduction in all experimental groups. Therefore, E-beam treatment may accelerate degradation. In our live micro-CT analyses, most volume reduction arose in the first four weeks after implantation and slowed between 4 and 20 weeks in all groups. E-beam groups showed greater volume reduction at every time point, which is consistent with the results by micro-CT analysis. Histology results suggest the biocompatibility of TCP/PCL composite filaments.

Original languageEnglish (US)
Article number273
JournalMicromachines
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2020

Keywords

  • 3D printing
  • Bone tissue engineering
  • Electron beam sterilization
  • Β-tricalcium phosphate/polycaprolactone (|3-TCP/PCL) composite

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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