The influence of dietary lipid composition on skeletal muscle mitochondria from mice following eight months of calorie restriction

Y. Chen, Kevork Hagopian, D. Bibus, J. M. Villalba, G. López-Lluch, P. Navas, Kyoungmi Kim, Jon J Ramsey

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Calorie restriction (CR) has been shown to decrease reactive oxygen species (ROS) production and retard aging in a variety of species. It has been proposed that alterations in membrane saturation are central to these actions of CR. As a step towards testing this theory, mice were assigned to 4 dietary groups (control and 3 CR groups) and fed AIN-93G diets at 95 % (control) or 60 % (CR) of ad libitum for 8 months. To manipulate membrane composition, the primary dietary fats for the CR groups were soybean oil (also used in the control diet), fish oil or lard. Skeletal muscle mitochondrial lipid composition, proton leak, and H2O2 production were measured. Phospholipid fatty acid composition in CR mice was altered in a manner that reflected the n-3 and n-6 fatty acid profiles of their respective dietary lipid sources. Dietary lipid composition did not alter proton leak kinetics between the CR groups. However, the capacity of mitochondrial complex III to produce ROS was decreased in the CR lard compared to the other CR groups. The results of this study indicate that dietary lipid composition can influence ROS production in muscle mitochondria of CR mice. It remains to be determined if lard or other dietary oils can maximize the CRinduced decreases in ROS production.

Original languageEnglish (US)
Pages (from-to)57-71
Number of pages15
JournalPhysiological Research
Volume63
Issue number1
StatePublished - 2014

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Muscle Mitochondrion
Reactive Oxygen Species
Skeletal Muscle
Lipids
Protons
Fatty Acids
Unsaturated Dietary Fats
Diet
Soybean Oil
Membranes
Fish Oils
Electron Transport Complex III
Dietary Fats
Phospholipids
Control Groups
lard

Keywords

  • Dietary lipids
  • Energy restriction
  • Mitochondria
  • Phospholipids
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)

Cite this

The influence of dietary lipid composition on skeletal muscle mitochondria from mice following eight months of calorie restriction. / Chen, Y.; Hagopian, Kevork; Bibus, D.; Villalba, J. M.; López-Lluch, G.; Navas, P.; Kim, Kyoungmi; Ramsey, Jon J.

In: Physiological Research, Vol. 63, No. 1, 2014, p. 57-71.

Research output: Contribution to journalArticle

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AU - Kim, Kyoungmi

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