Abstract
To investigate the role mitochondrial membrane lipids play in the actions of calorie restriction (CR), C57BL/6 mice were assigned to four groups (control and three 40% CR groups) and fed diets containing soybean oil (also in the control diet), fish oil, or lard. The fatty acid composition of the major mitochondrial phospholipid classes, proton leak, and H 2O 2 production were measured in muscle mitochondria following 1 month of CR. The results indicate that phospholipid fatty acids reflected the polyunsaturated fatty acid profile of the dietary lipid sources. Capacity for Complex I- and III-linked H 2O 2 production was decreased with CR, although there was no difference between CR groups. The CR lard group had lower proton leak than all other groups. The results indicate that a decreased degree of unsaturation in muscle mitochondrial membranes is not required for reduced H 2O 2 production with CR. However, dietary lipids do have some influence on proton leak with CR.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1121-1131 |
| Number of pages | 11 |
| Journal | Journals of Gerontology - Series A Biological Sciences and Medical Sciences |
| Volume | 67 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2012 |
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Keywords
- Energy restriction
- Fatty acids
- Phospholipids
- Proton leak
- Reactive oxygen species
ASJC Scopus subject areas
- Aging
- Geriatrics and Gerontology
- Medicine(all)
Cite this
The influence of dietary lipid composition on skeletal muscle mitochondria from mice following 1 month of calorie restriction. / Chen, Yana; Hagopian, Kevork; McDonald, Roger B.; Bibus, Douglas; López-Lluch, Guillermo; Villalba, José M.; Navas, Plácido; Ramsey, Jon J.
In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 67, No. 11, 11.2012, p. 1121-1131.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The influence of dietary lipid composition on skeletal muscle mitochondria from mice following 1 month of calorie restriction
AU - Chen, Yana
AU - Hagopian, Kevork
AU - McDonald, Roger B.
AU - Bibus, Douglas
AU - López-Lluch, Guillermo
AU - Villalba, José M.
AU - Navas, Plácido
AU - Ramsey, Jon J
PY - 2012/11
Y1 - 2012/11
N2 - To investigate the role mitochondrial membrane lipids play in the actions of calorie restriction (CR), C57BL/6 mice were assigned to four groups (control and three 40% CR groups) and fed diets containing soybean oil (also in the control diet), fish oil, or lard. The fatty acid composition of the major mitochondrial phospholipid classes, proton leak, and H 2O 2 production were measured in muscle mitochondria following 1 month of CR. The results indicate that phospholipid fatty acids reflected the polyunsaturated fatty acid profile of the dietary lipid sources. Capacity for Complex I- and III-linked H 2O 2 production was decreased with CR, although there was no difference between CR groups. The CR lard group had lower proton leak than all other groups. The results indicate that a decreased degree of unsaturation in muscle mitochondrial membranes is not required for reduced H 2O 2 production with CR. However, dietary lipids do have some influence on proton leak with CR.
AB - To investigate the role mitochondrial membrane lipids play in the actions of calorie restriction (CR), C57BL/6 mice were assigned to four groups (control and three 40% CR groups) and fed diets containing soybean oil (also in the control diet), fish oil, or lard. The fatty acid composition of the major mitochondrial phospholipid classes, proton leak, and H 2O 2 production were measured in muscle mitochondria following 1 month of CR. The results indicate that phospholipid fatty acids reflected the polyunsaturated fatty acid profile of the dietary lipid sources. Capacity for Complex I- and III-linked H 2O 2 production was decreased with CR, although there was no difference between CR groups. The CR lard group had lower proton leak than all other groups. The results indicate that a decreased degree of unsaturation in muscle mitochondrial membranes is not required for reduced H 2O 2 production with CR. However, dietary lipids do have some influence on proton leak with CR.
KW - Energy restriction
KW - Fatty acids
KW - Phospholipids
KW - Proton leak
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=84867509940&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867509940&partnerID=8YFLogxK
U2 - 10.1093/gerona/gls113
DO - 10.1093/gerona/gls113
M3 - Article
C2 - 22503990
AN - SCOPUS:84867509940
VL - 67
SP - 1121
EP - 1131
JO - The journals of gerontology. Series A, Biological sciences and medical sciences
JF - The journals of gerontology. Series A, Biological sciences and medical sciences
SN - 1079-5006
IS - 11
ER -