Abstract
To investigate the role mitochondrial membrane lipids play in the actions of calorie restriction (CR), C57BL/6 mice were assigned to four groups (control and three 40% CR groups) and fed diets containing soybean oil (also in the control diet), fish oil, or lard. The fatty acid composition of the major mitochondrial phospholipid classes, proton leak, and H 2O 2 production were measured in muscle mitochondria following 1 month of CR. The results indicate that phospholipid fatty acids reflected the polyunsaturated fatty acid profile of the dietary lipid sources. Capacity for Complex I- and III-linked H 2O 2 production was decreased with CR, although there was no difference between CR groups. The CR lard group had lower proton leak than all other groups. The results indicate that a decreased degree of unsaturation in muscle mitochondrial membranes is not required for reduced H 2O 2 production with CR. However, dietary lipids do have some influence on proton leak with CR.
Original language | English (US) |
---|---|
Pages (from-to) | 1121-1131 |
Number of pages | 11 |
Journal | Journals of Gerontology - Series A Biological Sciences and Medical Sciences |
Volume | 67 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2012 |
Keywords
- Energy restriction
- Fatty acids
- Phospholipids
- Proton leak
- Reactive oxygen species
ASJC Scopus subject areas
- Aging
- Geriatrics and Gerontology
- Medicine(all)