The influence of dietary lipid composition on skeletal muscle mitochondria from mice following 1 month of calorie restriction

Yana Chen, Kevork Hagopian, Roger B. McDonald, Douglas Bibus, Guillermo López-Lluch, José M. Villalba, Plácido Navas, Jon J Ramsey

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To investigate the role mitochondrial membrane lipids play in the actions of calorie restriction (CR), C57BL/6 mice were assigned to four groups (control and three 40% CR groups) and fed diets containing soybean oil (also in the control diet), fish oil, or lard. The fatty acid composition of the major mitochondrial phospholipid classes, proton leak, and H 2O 2 production were measured in muscle mitochondria following 1 month of CR. The results indicate that phospholipid fatty acids reflected the polyunsaturated fatty acid profile of the dietary lipid sources. Capacity for Complex I- and III-linked H 2O 2 production was decreased with CR, although there was no difference between CR groups. The CR lard group had lower proton leak than all other groups. The results indicate that a decreased degree of unsaturation in muscle mitochondrial membranes is not required for reduced H 2O 2 production with CR. However, dietary lipids do have some influence on proton leak with CR.

Original languageEnglish (US)
Pages (from-to)1121-1131
Number of pages11
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume67
Issue number11
DOIs
StatePublished - Nov 2012

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Muscle Mitochondrion
Protons
Skeletal Muscle
Mitochondrial Membranes
Lipids
Phospholipids
Fatty Acids
Diet
Soybean Oil
Fish Oils
Membrane Lipids
Unsaturated Fatty Acids
Inbred C57BL Mouse
Muscles
Control Groups
lard

Keywords

  • Energy restriction
  • Fatty acids
  • Phospholipids
  • Proton leak
  • Reactive oxygen species

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology
  • Medicine(all)

Cite this

The influence of dietary lipid composition on skeletal muscle mitochondria from mice following 1 month of calorie restriction. / Chen, Yana; Hagopian, Kevork; McDonald, Roger B.; Bibus, Douglas; López-Lluch, Guillermo; Villalba, José M.; Navas, Plácido; Ramsey, Jon J.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 67, No. 11, 11.2012, p. 1121-1131.

Research output: Contribution to journalArticle

Chen, Yana ; Hagopian, Kevork ; McDonald, Roger B. ; Bibus, Douglas ; López-Lluch, Guillermo ; Villalba, José M. ; Navas, Plácido ; Ramsey, Jon J. / The influence of dietary lipid composition on skeletal muscle mitochondria from mice following 1 month of calorie restriction. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2012 ; Vol. 67, No. 11. pp. 1121-1131.
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