The influence of dietary lipid composition on liver mitochondria from mice following 1 month of calorie restriction

Yana Chen, Kevork Hagopian, Douglas Bibus, José M. Villalba, Guillermo L. Opez-Lluch, Placido Navas, Kyoungmi Kim, Roger B. McDonald, Jon J Ramsey

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


To investigate the role mitochondrial membrane lipids play in the actions of CR (calorie restriction), C57BL/6 mice were assigned to four groups (control and three 40% CR groups) and the CR groups were fed diets containing soya bean oil (also in the control diet), fish oil or lard. The fatty acid composition of the major mitochondrial phospholipid classes, proton leak and H2O2 production were measured in liver mitochondria following 1 month of CR. The results indicate that mitochondrial phospholipid fatty acids reflect the PUFA (polyunsaturated fatty acid) profile of the dietary lipid sources. CR significantly decreased the capacity of ROS (reactive oxygen species) production by Complex III but did not markedly alter proton leak and ETC (electron transport chain) enzyme activities. Within the CR regimens, the CR-fish group had decreased ROS production by both Complexes I and III, and increased proton leak when compared with the other CR groups. The CR-lard group showed the lowest proton leak compared with the other CR groups. The ETC enzyme activity measurements in the CR regimens showed that Complex I activity was decreased in both the CR-fish and CR-lard groups. Moreover, the CR-fish group also had lower Complex II activity compared with the other CR groups. These results indicate that dietary lipid composition does influence liver mitochondrial phospholipid composition, ROS production, proton leak and ETC enzyme activities in CR animals.

Original languageEnglish (US)
Article numberA9
Pages (from-to)83-95
Number of pages13
JournalBioscience Reports
Issue number1
StatePublished - Feb 2013


  • Aging
  • Energy restriction
  • Mitochondria
  • Oxidative stress phospholipids
  • Proton leak

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology


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