Abstract
Complexes of murine monoclonal anti-DNP IgE and DNP-OVA interact with murine B cells` to stimulate expression of cell surface Ia antigens. Enhanced membrane expression of class II MHC antigens was accompanied by a threefold increase of I-A and I-E transcripts, as measured by Northern blot. Peak accumulation of Ia mRNA were detected after 6 hr of incubation with IgE-antigen complexes and returned to control levels after 12 hr of incubation. Hence, induction of Ia mRNA by IgE-antigen complexes was compatible with cell surface Ia expression, both quantitatively and with regard to the time frame. The Ia-inductive effects of both IL-4 and IgE-antigen complexes were inhibited by cycloheximide and actinomycin-D. However, whereas actinomycin-D and cycloheximide blocked IL-4 induction of Fcε{lunate}RII expression, inhibition of transcription or protein synthesis did not abrogate the increased expression of Fcε{lunate}R associated with IgE-antigen complexes. These results suggest that the IgE-antigen-induction of B cell Ia expression follows from activation of transcription and de novo synthesis of Ia antigens.
Original language | English (US) |
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Pages (from-to) | 198-208 |
Number of pages | 11 |
Journal | Cellular Immunology |
Volume | 128 |
Issue number | 1 |
DOIs | |
State | Published - 1990 |
ASJC Scopus subject areas
- Cell Biology
- Immunology