The inducing role and molecular basis of bursal hexapeptide (BHP) on avian immature B cell

Xiu Li Feng, Yang Zheng, Shan Shan Hao, Guang Fang Zhou, Pu Yan Chen

Research output: Contribution to journalArticle

Abstract

Background: The Bursa of Fabricius is an acknowledged central humoral immune organ unique to birds, which provides an ideal research model on the immature B cell development. Objective: In this article, our motivation is to study the role on sIgM and establish the molecular basis and functional processes of Bursal Hexapeptide (BHP) in avian immature B cells DT40 cell lines. Methods: In this article, we detected the expressions of sIgM mRNA with qPCR in DT40 cells with BHP treatment, and investigated the gene expression profiles of BHP-treated DT40 cells, employing microarray analyses. Also, to validate the differentially expressed genes, we performed KEGG pathway and Gene Ontology analysis in the BHP-treated DT40 cells. Finally, we comparatively analyzed the similar regulated genes and their involved immune functional processes between DT40 cell and mouse immature B cell line WEHI231 cell with BHP treatment. Results: Following the proposed framework, we proved that the BHP enhanced the mRNA expression levels of IgM in DT40 cells, and induced 460 upregulated genes and 460 downregulated genes in BHP-treated DT40 cells. The pathway analysis showed that the differentially regulated genes in DT40 cell line with BHP treatment were involved in 12 enrichment pathways, in which Toll-like receptor signaling pathway was the vital pathways, and cytokine-cytokine receptor interaction and Jak-STAT signaling pathway were another two important pathways in BHP-treated DT40 cells. Moreover, BHP induced the immune related biological processes in BHP-treated DT40 cells, including T cell related, cytokine related, lymphocyte related, and innate immune response GO terms. Finally, the comparatively analysis showed that there were two downregulated genes GATA3 and IFNG to be found co-existed among the differentially expressed genes in BHP-treated DT40 cell and WEHI231 cells, which shared some same immune related functional processes in both cell lines. Conclusion: After the applying the framework, we proved the inducing roles and the gene expression profiles of BHP on avian immature B cells, and verified some molecular basis from the KEGG and GO analysis. These results provided the insight for mechanism on immature B cell differentiation, and offer the essential direction for the vaccine improvement.

Original languageEnglish (US)
Pages (from-to)348-356
Number of pages9
JournalProtein and Peptide Letters
Volume26
Issue number5
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

B-Lymphoid Precursor Cells
Genes
Cells
Cell Line
Gene expression
Transcriptome
Cytokines
Down-Regulation
Messenger RNA
Cytokine Receptors
T-cells
Lymphocytes
Tissue Array Analysis
Toll-Like Receptors
Bursa of Fabricius
Birds
Biological Phenomena
Microarrays
Gene Ontology
Immunoglobulin M

Keywords

  • Bursal Hexapeptide (BHP)
  • IgM
  • Immature B cell
  • Immune functional processes
  • Pathways
  • Toll-like receptor signaling pathway

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

Cite this

The inducing role and molecular basis of bursal hexapeptide (BHP) on avian immature B cell. / Feng, Xiu Li; Zheng, Yang; Hao, Shan Shan; Zhou, Guang Fang; Chen, Pu Yan.

In: Protein and Peptide Letters, Vol. 26, No. 5, 01.01.2019, p. 348-356.

Research output: Contribution to journalArticle

Feng, Xiu Li ; Zheng, Yang ; Hao, Shan Shan ; Zhou, Guang Fang ; Chen, Pu Yan. / The inducing role and molecular basis of bursal hexapeptide (BHP) on avian immature B cell. In: Protein and Peptide Letters. 2019 ; Vol. 26, No. 5. pp. 348-356.
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AU - Chen, Pu Yan

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