The increase in radioresistance of Chinese hamster cells cultured as spheroids is correlated to changes in nuclear morphology

D. J. Gordon, A. E. Milner, R. P. Beany, D. J. Grdina, Andrew T M Vaughan

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Chinese hamster V79 cells grown as spheroids in roller culture are more radioresistant than those grown as monolayers. The supercoiled structure of chromatin, as salt-extracted nucleotides, has been examined using flow cytometry. Irradiated viable cells from spheroid culture contain restraints to supercoil relaxation that are absent in monolayer cells. Further analysis of the chromatin organization from each growth form shows that the radioresistant spheroid cells contain a DNA-protein matrix that is more resistant to detergent-induced degradation. The increase in structural integrity may be due to the retention of a 55-60 kDa protein that is apparent in the nucleoids of spheroid, but not monolayer cells. The increase in structural integrity of the spheroid cells may explain their greater radioresistance by providing a more stable platform for high-fidelity DNA damage repair.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalRadiation Research
Volume121
Issue number2
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

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