The in vitro synthesis and degradation of prostaglandins during the development of bleomycin-induced pulmonary fibrosis in hamsters1-3

D. B. Chandler, S. N. Giri, Z. Chen, D. M. Hyde

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Subsequent to optimization of conditions for enzyme assay, we examined the in vitro synthesis and degradation of prostaglandins by the lung during the devel opment of bleomycin-induced pulmonary fibrosis in hamsters. It was found that the microsomal protein content on a per lung basis was significantly increased to 144, 129, 134, and 121% of control (2.3 mg protein/lung) at 4, 7, 14 and 21 days post-treatment, respectively. The synthesis of PGD2 was significantly elevated to 10.2, 10.8, and 12.5 moles/lung at 7, 21 and 28 days, respectively, as compared to the control value of 5.6 moles/lung. Significant increases in PGF synthesis from the control value of 3.3 moles/lung to 5.2, 8.2 and 5.5 moles/lung were found at 4, 7 and 21 days post-treatment, respectively. The synthesis of PGE2 also showed significant increases above above the control value of 6.1 moles/lung to 10.5, 12.2 and 11.0 nmoles/lung at 7, 21 and 28 days post-treatment, respectively. Similarly, the synthesis of 6-keto-PGF was significantly increased to 7.4, 7.5 and 8.6 moles/lung at 7, 21 and 28 days post-treatment, respectively, as compared to the control value of 4.4 moles/lung. The synthesis of TxB was also significantly increased from the control value of 3.9 nmoles/lung to 7.5 and 6.4 moles/lung at 7 and 21 days post-treatment, respectively. Accompanying the increased synthesis of prostaglandins in general, the in vitro degradation of PGF was significantly increased from the control v of 71.1 nmoles/lung to 173.5, 131.7 and 143.3 nmoles/lung at at 2, 4 and 7 days after bleomycin treatment, respectively. We conclude that bleomycin-induced pulmonary fibrosis leads to changes in prostaglandin synthesis and degradation possibly as a result of an accompanying inflammatorv response and resident cellular oroliferation.

Original languageEnglish (US)
Pages (from-to)11-31
Number of pages21
JournalProstaglandins, Leukotrienes and Medicine
Volume11
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

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