The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice

Milena Menegola, Eliana Clark, James Trimmer

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To gain insights into the phenotype of voltage-gated potassium (Kv)1.1 and Kv4.2 knockout mice, we used immunohistochemistry to analyze the expression of component principal or α subunits and auxiliary subunits of neuronal Kv channels in knockout mouse brains. Genetic ablation of the Kv1.1 α subunit did not result in compensatory changes in the expression levels or subcellular distribution of related ion channel subunits in hippocampal medial perforant path and mossy fiber nerve terminals, where high levels of Kv1.1 are normally expressed. Genetic ablation of the Kv4.2 α subunit did not result in altered neuronal cytoarchitecture of the hippocampus. Although Kv4.2 knockout mice did not exhibit compensatory changes in the expression levels or subcellular distribution of the related Kv4.3 α subunit, we found dramatic decreases in the cellular and subcellular expression of specific Kv channel interacting proteins (KChIPs) that reflected their degree of association and colocalization with Kv4.2 in wild-type mouse and rat brains. These studies highlight the insights that can be gained by performing detailed immunohistochemical analyses of Kv channel knockout mouse brains.

Original languageEnglish (US)
Pages (from-to)142-149
Number of pages8
JournalEpilepsia
Volume53
Issue numberSUPPL. 1
DOIs
StatePublished - Jun 2012

Fingerprint

Knockout Mice
Phenotype
Kv Channel-Interacting Proteins
Brain
Perforant Pathway
Principal Component Analysis
Ion Channels
Nerve Fibers
Hippocampus
Potassium
Immunohistochemistry

Keywords

  • Antibodies
  • Gene expression
  • Hippocampus
  • Potassium channel
  • Seizures
  • Subcellular localization

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice. / Menegola, Milena; Clark, Eliana; Trimmer, James.

In: Epilepsia, Vol. 53, No. SUPPL. 1, 06.2012, p. 142-149.

Research output: Contribution to journalArticle

Menegola, Milena ; Clark, Eliana ; Trimmer, James. / The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice. In: Epilepsia. 2012 ; Vol. 53, No. SUPPL. 1. pp. 142-149.
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