TY - JOUR
T1 - The Impact of Aging, Calorie Restriction and Dietary Fat on Autophagy Markers and Mitochondrial Ultrastructure and Dynamics in Mouse Skeletal Muscle
AU - Gutiérrez-Casado, Elena
AU - Khraiwesh, Husam
AU - López-Domínguez, José A.
AU - Montero-Guisado, Jesús
AU - López-Lluch, Guillermo
AU - Navas, Plácido
AU - de Cabo, Rafael
AU - Ramsey, Jon J
AU - González-Reyes, José A.
AU - Villalba, José M.
PY - 2019/5/16
Y1 - 2019/5/16
N2 - Loss of skeletal muscle mass and function is a hallmark of aging. This phenomenon has been related to a dysregulation of mitochondrial function and proteostasis. Calorie restriction (CR) has been demonstrated to delay aging and preserve function until late in life, particularly in muscle. Recently, we reported the type of dietary fat plays an important role in determining life span extension with 40% CR in male mice. In these conditions, lard fed mice showed an increased longevity compared to mice fed soybean or fish oils. In this article, we analyze the effect of 40% CR on muscle mitochondrial mass, autophagy, and mitochondrial dynamics markers in mice fed these diets. In CR fed animals, lard preserved muscle fibers structure, mitochondrial ultrastructure, and fission/fusion dynamics and autophagy, not only compared to control animals, but also compared with CR mice fed soybean and fish oils as dietary fat. We focus our discussion on dietary fatty acid saturation degree as an essential predictor of life span extension in CR mice.
AB - Loss of skeletal muscle mass and function is a hallmark of aging. This phenomenon has been related to a dysregulation of mitochondrial function and proteostasis. Calorie restriction (CR) has been demonstrated to delay aging and preserve function until late in life, particularly in muscle. Recently, we reported the type of dietary fat plays an important role in determining life span extension with 40% CR in male mice. In these conditions, lard fed mice showed an increased longevity compared to mice fed soybean or fish oils. In this article, we analyze the effect of 40% CR on muscle mitochondrial mass, autophagy, and mitochondrial dynamics markers in mice fed these diets. In CR fed animals, lard preserved muscle fibers structure, mitochondrial ultrastructure, and fission/fusion dynamics and autophagy, not only compared to control animals, but also compared with CR mice fed soybean and fish oils as dietary fat. We focus our discussion on dietary fatty acid saturation degree as an essential predictor of life span extension in CR mice.
KW - Caloric restriction
KW - Dietary fat
KW - Mice
KW - Mitochondria
KW - Muscles
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UR - http://www.scopus.com/inward/citedby.url?scp=85066918823&partnerID=8YFLogxK
U2 - 10.1093/gerona/gly161
DO - 10.1093/gerona/gly161
M3 - Article
C2 - 30010806
AN - SCOPUS:85066918823
VL - 74
SP - 760
EP - 769
JO - The journals of gerontology. Series A, Biological sciences and medical sciences
JF - The journals of gerontology. Series A, Biological sciences and medical sciences
SN - 1079-5006
IS - 6
ER -