The immunomodulatory peptide bursopentin (BP5) enhances proliferation and induces sIgM expression in DT40 cells

Xiang Bo Ji, Jun Luo, Xiuli Feng, Qiu Liang Xu, Teng Man, Dong Zhao, Xin Feng Li, Gai Ping Zhang, Pu Yan Chen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: In the recent past, many studies have been focused on extracts of BF and multiple biologically active factors and their effects on humoral immune system in chickens and birds. However, the mechanism of those immunomodulatory peptides on the B lineage cells proliferation and antibody production in chicken is fairly unknown. DT40 cell line, an avian leucosis virus-induced chicken pre-B cell line, expresses immunoglobulin M (IgM) isotype B cell reporter in the plasma membrane. There are many evidences suggesting that DT40 cells are best characterized as a bursal stem cell line. Because of the unique characteristics of DT40 cell line, it has been widely used to observe biological processes of pre-B lymphocyte cell within living cells. Methods: The chicken B cell line DT40 was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium and cytotoxicity was studied. Also, effect of BP5 on cell proliferation and cell cycle distribution of DT40 cells was studied. Also, the effect of BP5 on sIgM mRNA expression was studied by using real-time PCR. Objectives: To investigat the effects of Bursopentin (Cys-Lys-Arg-Val-Tyr, BP5) on a chicken promyelocyte cell line DT40, assays of cell proliferation, cell cycle distribution, detection of surface immunoglobulin G (sIgM) mRNA expression and gene microarray analysis were performed. Results: The results showed that BP5 displayed concentration-dependent effects on the proliferation, cell cycle, and sIgM mRNA expression in DT40 cells. And the analysis of expression profiles identified a signature set of 3022 genes (1254 up regulated genes, 1762 down regulated genes), which clearly discriminated the BP5-treated DT40 cells from control with high certainty (P≤0.02). The results of microarray analysis were confirmed by quantitative reverse transcription-polymerase chain reaction for 12 of the differentially expressed genes. Conclusion: Theses findings showed the immuno-activity effect of BP5 on B lymphocyte and indicated that BP5 treatment regulated eight signaling pathways, in which Toll-like signaling pathway was the most significant enrichment pathway.

Original languageEnglish (US)
Pages (from-to)1292-1302
Number of pages11
JournalAfrican health sciences
Volume18
Issue number4
DOIs
StatePublished - Dec 1 2018
Externally publishedYes

Fingerprint

Chickens
Cell Line
Peptides
B-Lymphoid Precursor Cells
Cell Cycle
B-Lymphocytes
Cell Proliferation
Microarray Analysis
Messenger RNA
Genes
Avian Leukosis Virus
Biological Phenomena
Granulocyte Precursor Cells
B-Cell Antigen Receptors
Immunoglobulin Isotypes
Reverse Transcription
Birds
Antibody Formation
Immunoglobulin M
Real-Time Polymerase Chain Reaction

Keywords

  • Bursopentin (BP5)
  • Cell cycle
  • DT40 cell
  • gene microarray
  • Proliferation
  • sIgM

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The immunomodulatory peptide bursopentin (BP5) enhances proliferation and induces sIgM expression in DT40 cells. / Ji, Xiang Bo; Luo, Jun; Feng, Xiuli; Xu, Qiu Liang; Man, Teng; Zhao, Dong; Li, Xin Feng; Zhang, Gai Ping; Chen, Pu Yan.

In: African health sciences, Vol. 18, No. 4, 01.12.2018, p. 1292-1302.

Research output: Contribution to journalArticle

Ji, Xiang Bo ; Luo, Jun ; Feng, Xiuli ; Xu, Qiu Liang ; Man, Teng ; Zhao, Dong ; Li, Xin Feng ; Zhang, Gai Ping ; Chen, Pu Yan. / The immunomodulatory peptide bursopentin (BP5) enhances proliferation and induces sIgM expression in DT40 cells. In: African health sciences. 2018 ; Vol. 18, No. 4. pp. 1292-1302.
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AU - Luo, Jun

AU - Feng, Xiuli

AU - Xu, Qiu Liang

AU - Man, Teng

AU - Zhao, Dong

AU - Li, Xin Feng

AU - Zhang, Gai Ping

AU - Chen, Pu Yan

PY - 2018/12/1

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N2 - Background: In the recent past, many studies have been focused on extracts of BF and multiple biologically active factors and their effects on humoral immune system in chickens and birds. However, the mechanism of those immunomodulatory peptides on the B lineage cells proliferation and antibody production in chicken is fairly unknown. DT40 cell line, an avian leucosis virus-induced chicken pre-B cell line, expresses immunoglobulin M (IgM) isotype B cell reporter in the plasma membrane. There are many evidences suggesting that DT40 cells are best characterized as a bursal stem cell line. Because of the unique characteristics of DT40 cell line, it has been widely used to observe biological processes of pre-B lymphocyte cell within living cells. Methods: The chicken B cell line DT40 was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium and cytotoxicity was studied. Also, effect of BP5 on cell proliferation and cell cycle distribution of DT40 cells was studied. Also, the effect of BP5 on sIgM mRNA expression was studied by using real-time PCR. Objectives: To investigat the effects of Bursopentin (Cys-Lys-Arg-Val-Tyr, BP5) on a chicken promyelocyte cell line DT40, assays of cell proliferation, cell cycle distribution, detection of surface immunoglobulin G (sIgM) mRNA expression and gene microarray analysis were performed. Results: The results showed that BP5 displayed concentration-dependent effects on the proliferation, cell cycle, and sIgM mRNA expression in DT40 cells. And the analysis of expression profiles identified a signature set of 3022 genes (1254 up regulated genes, 1762 down regulated genes), which clearly discriminated the BP5-treated DT40 cells from control with high certainty (P≤0.02). The results of microarray analysis were confirmed by quantitative reverse transcription-polymerase chain reaction for 12 of the differentially expressed genes. Conclusion: Theses findings showed the immuno-activity effect of BP5 on B lymphocyte and indicated that BP5 treatment regulated eight signaling pathways, in which Toll-like signaling pathway was the most significant enrichment pathway.

AB - Background: In the recent past, many studies have been focused on extracts of BF and multiple biologically active factors and their effects on humoral immune system in chickens and birds. However, the mechanism of those immunomodulatory peptides on the B lineage cells proliferation and antibody production in chicken is fairly unknown. DT40 cell line, an avian leucosis virus-induced chicken pre-B cell line, expresses immunoglobulin M (IgM) isotype B cell reporter in the plasma membrane. There are many evidences suggesting that DT40 cells are best characterized as a bursal stem cell line. Because of the unique characteristics of DT40 cell line, it has been widely used to observe biological processes of pre-B lymphocyte cell within living cells. Methods: The chicken B cell line DT40 was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium and cytotoxicity was studied. Also, effect of BP5 on cell proliferation and cell cycle distribution of DT40 cells was studied. Also, the effect of BP5 on sIgM mRNA expression was studied by using real-time PCR. Objectives: To investigat the effects of Bursopentin (Cys-Lys-Arg-Val-Tyr, BP5) on a chicken promyelocyte cell line DT40, assays of cell proliferation, cell cycle distribution, detection of surface immunoglobulin G (sIgM) mRNA expression and gene microarray analysis were performed. Results: The results showed that BP5 displayed concentration-dependent effects on the proliferation, cell cycle, and sIgM mRNA expression in DT40 cells. And the analysis of expression profiles identified a signature set of 3022 genes (1254 up regulated genes, 1762 down regulated genes), which clearly discriminated the BP5-treated DT40 cells from control with high certainty (P≤0.02). The results of microarray analysis were confirmed by quantitative reverse transcription-polymerase chain reaction for 12 of the differentially expressed genes. Conclusion: Theses findings showed the immuno-activity effect of BP5 on B lymphocyte and indicated that BP5 treatment regulated eight signaling pathways, in which Toll-like signaling pathway was the most significant enrichment pathway.

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