The immunohistochemical demonstration of noradrenergic neurons in the rat brain: The use of homologous antiserum to dopamine-β-hydroxylase

Reinhard Grzanna, John Morrison, Joseph T. Coyle, Mark E. Molliver

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

The immunohistochemical localization of dopamine-β-hydroxylase (DBH) in central noradrenergic neurons of the rat is greatly improved by the use of homologous antiserum (i.e. antibody against purified rat DBH). This antiserum, which has a several thousand-fold greater affinity for rat DBH than does heterologous antibody, is used at a 1:500 dilution with low concentrations of detergent. This method applied to paraformaldehyde-perfused tissue consistently yields highly sensitive and selective staining with excellent cytologic preservation of fine neuronal processes.

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalNeuroscience Letters
Volume4
Issue number3-4
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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