The immune response of carp to Trypanoplasma borreli: Kinetics of immune gene expression and polyclonal lymphocyte activation

Jeroen Saeij, Beitske J. De Vries, Geert F. Wiegertjes

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Although Trypanoplasma borreli induces the production of non-specific antibodies, survival of infection is associated with the production of T. borreli specific antibodies, able to lyse this parasite in the presence of complement. During the lag phase of this acquired immune response, innate immune mechanisms must limit multiplication of T. borreli. A heat-labile fraction of T. borreli together with CpG motifs in the DNA of this parasite are responsible for the induction of nitric oxide (NO) and probably also for the induction of expression of the inflammatory cytokines tumor necrosis factor (TNF)α and interleukin (IL)-1β by carp phagocytes in vitro. In the signal transduction pathway leading to activation of phagocytes, protein tyrosine kinase and protein kinase C are involved and probably collaborate in activation of the transcription factor nuclear factor (NF)-κB. In vivo, carp intraperitoneally injected with T. borreli up-regulate expression of TNFα, IL-1β and mRNAs for acute phase response proteins (complement factor 3, serum amyloid A and alpha-2-macroglobulin).

Original languageEnglish (US)
Pages (from-to)859-874
Number of pages16
JournalDevelopmental and Comparative Immunology
Volume27
Issue number10
DOIs
StatePublished - Dec 2003
Externally publishedYes

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Carps
Phagocytes
Lymphocyte Activation
Interleukin-1
Innate Immunity
Parasites
Tumor Necrosis Factor-alpha
alpha-Macroglobulins
Gene Expression
Serum Amyloid A Protein
Acute-Phase Reaction
Nucleotide Motifs
Complement C3
Complement Factor B
Acute-Phase Proteins
Antibodies
Protein-Tyrosine Kinases
Protein Kinase C
Signal Transduction
Nitric Oxide

Keywords

  • Carp
  • Cytokines
  • Parasite immunity
  • Signal transduction
  • Trypanosomes

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

The immune response of carp to Trypanoplasma borreli : Kinetics of immune gene expression and polyclonal lymphocyte activation. / Saeij, Jeroen; De Vries, Beitske J.; Wiegertjes, Geert F.

In: Developmental and Comparative Immunology, Vol. 27, No. 10, 12.2003, p. 859-874.

Research output: Contribution to journalArticle

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