The hydantoin lesions formed from oxidation of 7,8-dihydro-8-oxoguanine are potent sources of replication errors in vivo

Paul Henderson, James C. Delaney, James G. Muller, William L. Neeley, Steven R. Tannenbaum, Cynthia J. Burrows, John M. Essigmann

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

Single-stranded DNA genomes have been constructed that site-specifically contain the 7,8-dihydro-8-oxo-2′-deoxyguanine (8-oxoG) oxidation products guanidinohydantoin (Gh) and the two stable stereoisomers of spiroiminodihydantoin (Sp1 and Sp2). The circular viral genomes were transfected into wild-type AB1157 Escherichia coli, and the efficiency of lesion bypass by DNA polymerase(s) was assessed. Viral progeny were analyzed for mutation frequency and type using the recently developed restriction endonuclease and postlabeling (REAP) assay. Gh was bypassed nearly as efficiently as the parent 8-oxoG but was highly mutagenic, causing almost exclusive G → C transversions. The stereoisomers Sp1 and Sp2 were, in comparison, much stronger blocks to DNA polymerase extension and caused a mixture of G → T and G → C transversions. The ratio of G → T to G → C mutations for each Sp lesion was dependent on the stereochemical configuration of the base. All observed mutation frequencies were at least an order of magnitude higher than those caused by 8-oxoG. Were these lesions to be formed in vivo, our data show that they are absolutely miscoding and may be refractory to repair after translesion synthesis.

Original languageEnglish (US)
Pages (from-to)9257-9262
Number of pages6
JournalBiochemistry
Volume42
Issue number31
DOIs
StatePublished - Aug 12 2003
Externally publishedYes

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Hydantoins
Stereoisomerism
Mutation Rate
DNA-Directed DNA Polymerase
Research Design
Genes
Oxidation
Viral Genome
Single-Stranded DNA
DNA Restriction Enzymes
Refractory materials
Escherichia coli
Assays
Repair
Genome
Mutation
7,8-dihydro-8-oxoguanine
guanidinohydantoin
spiroiminodihydantoin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Henderson, P., Delaney, J. C., Muller, J. G., Neeley, W. L., Tannenbaum, S. R., Burrows, C. J., & Essigmann, J. M. (2003). The hydantoin lesions formed from oxidation of 7,8-dihydro-8-oxoguanine are potent sources of replication errors in vivo. Biochemistry, 42(31), 9257-9262. https://doi.org/10.1021/bi0347252

The hydantoin lesions formed from oxidation of 7,8-dihydro-8-oxoguanine are potent sources of replication errors in vivo. / Henderson, Paul; Delaney, James C.; Muller, James G.; Neeley, William L.; Tannenbaum, Steven R.; Burrows, Cynthia J.; Essigmann, John M.

In: Biochemistry, Vol. 42, No. 31, 12.08.2003, p. 9257-9262.

Research output: Contribution to journalArticle

Henderson, P, Delaney, JC, Muller, JG, Neeley, WL, Tannenbaum, SR, Burrows, CJ & Essigmann, JM 2003, 'The hydantoin lesions formed from oxidation of 7,8-dihydro-8-oxoguanine are potent sources of replication errors in vivo', Biochemistry, vol. 42, no. 31, pp. 9257-9262. https://doi.org/10.1021/bi0347252
Henderson, Paul ; Delaney, James C. ; Muller, James G. ; Neeley, William L. ; Tannenbaum, Steven R. ; Burrows, Cynthia J. ; Essigmann, John M. / The hydantoin lesions formed from oxidation of 7,8-dihydro-8-oxoguanine are potent sources of replication errors in vivo. In: Biochemistry. 2003 ; Vol. 42, No. 31. pp. 9257-9262.
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