The Homotetrameric Kinesin-5 KLP61F Preferentially Crosslinks Microtubules into Antiparallel Orientations

Siet M J L van den Wildenberg, Li Tao, Lukas C. Kapitein, Christoph F. Schmidt, Jonathan M. Scholey, Erwin J G Peterman

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

The segregation of genetic material during mitosis is coordinated by the mitotic spindle, whose action depends upon the polarity patterns of its microtubules (MTs) [1, 2]. Homotetrameric mitotic kinesin-5 motors can crosslink and slide adjacent spindle MTs [3-11], but it is unknown whether they or other motors contribute to establishing these MT polarity patterns. Here, we explored whether the Drosophila embryo kinesin-5 KLP61F, which plausibly crosslinks both parallel and antiparallel MTs [7, 12], displays a preference for parallel or antiparallel MT orientation. In motility assays, KLP61F was observed to crosslink and slide adjacent MTs, as predicted. Remarkably, KLP61F displayed a 3-fold higher preference for crosslinking MTs in the antiparallel orientation. This polarity preference was observed in the presence of ADP or ATP plus AMPPNP, but not AMPPNP alone, which induces instantaneous rigor binding. Also, a purified motorless tetramer containing the C-terminal tail domains displayed an antiparallel orientation preference, confirming that motor activity is not required. The results suggest that, during morphogenesis of the Drosophila embryo mitotic spindle, KLP61F's crosslinking and sliding activities could facilitate the gradual accumulation of KLP61F within antiparallel interpolar MTs at the equator, where the motor could generate force to drive poleward flux and pole-pole separation.

Original languageEnglish (US)
Pages (from-to)1860-1864
Number of pages5
JournalCurrent Biology
Volume18
Issue number23
DOIs
StatePublished - Dec 9 2008

Keywords

  • CELLBIO

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    van den Wildenberg, S. M. J. L., Tao, L., Kapitein, L. C., Schmidt, C. F., Scholey, J. M., & Peterman, E. J. G. (2008). The Homotetrameric Kinesin-5 KLP61F Preferentially Crosslinks Microtubules into Antiparallel Orientations. Current Biology, 18(23), 1860-1864. https://doi.org/10.1016/j.cub.2008.10.026