The heterotrimeric motor protein kinesin-II localizes to the midpiece and flagellum of sea urchin and sand dollar sperm

John H. Henson, Douglas G. Cole, Colleen D. Roesener, Stephanie Capuano, Robert J. Mendola, Jonathan M. Scholey

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

We have utilized immunoblotting and light microscopic immunofluorescent staining methods to examine the expression and localization of sea urchin kinesin-II, a heterotrimeric plus end-directed microtubule motor protein (previously referred to as KRP((85/95))), in sea urchin and sand dollar sperm. We demonstrate the presence of the 85 K and 115 K subunits of kinesin- II in sperm and localize these proteins to the sperm flagella and midpiece. The kinesin-II localization pattern is punctate and discontinuous, and in the flagella it is quite distinct from the continuous labeling present in sperm labeled with anti-flagellar dynein. The kinesin-II staining is largely insensitive to prefixation detergent extraction, suggesting that it is not associated with membranous elements in the sperm. In the midpiece the kinesin-II staining is similar to the pattern present in sperm labeled with an anti-centrosomal antibody. To our knowledge, this is the first localization of kinesin-like proteins in mature sperm and corroborates the recent identification and localization of kinesin-like proteins in the flagella and basal body of the unicellular green alga Chlamydomonas. We hypothesize that kinesin-II in the sperm may play functional roles in intraflagellar transport and/or the formation of flagella during spermatogenesis.

Original languageEnglish (US)
Pages (from-to)29-37
Number of pages9
JournalCell Motility and the Cytoskeleton
Volume38
Issue number1
DOIs
StatePublished - 1997

Keywords

  • Kinesin-II
  • Sand dollar sperm
  • Sea urchin

ASJC Scopus subject areas

  • Cell Biology

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