The genomic RNA of diabetogenic encephalomyocarditis virus: characterization and molecular cloning

George W. Jordan, Stuart H Cohen, Satya Dandekar, Kurt M. VandenBrink

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The RNA of a diabetogenic variant of encephalomyocarditis (EMC) virus (D variant, ifp- phenotype) and a nondiabetogenic variant of EMC virus (B variant, ifp+ phenotype) which were derived from the same virus stock (M strain) have been compared. The size of both genomes is estimated at 7.7 kb. The poly(C) tract of EMC-D is estimated at 144 bases, whereas that of EMC-B is 141 bases in length. The untranslated 5′ terminal 103 nucleotides are identical for B and D with preservation of a stable terminal hairpin structure. The entire open reading frame of both variants has been cloned and the restriction maps of 12 different enzymes are identical. These maps were compared to a computer-generated restriction map of another strain of EMC virus which has been cloned and sequenced by Palmenberg et al. (1984, Nucleic Acids Res. 12, 2969-2985). Approximately 50% of the restriction sites of the B and D variants had similar locations in this strain of EMC. We conclude that significant genetic variation exists between the M strain (B and D variants) and the Palmenberg strain of EMC virus. However, the B and D variants are very similar at the molecular level and comparison of their nucleotide sequences will be necessary to reveal the basis for their different biological properties.

Original languageEnglish (US)
Pages (from-to)120-125
Number of pages6
JournalVirology
Volume159
Issue number1
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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