The genome of the intracellular bacterium of the coastal bivalve, Solemya velum: A blueprint for thriving in and out of symbiosis

Oleg Dmytrenko, Shelbi L. Russell, Wesley T. Loo, Kristina M. Fontanez, Li Liao, Guus Roeselers, Raghav Sharma, Frank J. Stewart, Irene L G Newton, Tanja Woyke, Dongying Wu, Jenna Morgan Lang, Jonathan A Eisen, Colleen M. Cavanaugh

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Background: Symbioses between chemoautotrophic bacteria and marine invertebrates are rare examples of living systems that are virtually independent of photosynthetic primary production. These associations have evolved multiple times in marine habitats, such as deep-sea hydrothermal vents and reducing sediments, characterized by steep gradients of oxygen and reduced chemicals. Due to difficulties associated with maintaining these symbioses in the laboratory and culturing the symbiotic bacteria, studies of chemosynthetic symbioses rely heavily on culture independent methods. The symbiosis between the coastal bivalve, Solemya velum, and its intracellular symbiont is a model for chemosynthetic symbioses given its accessibility in intertidal environments and the ability to maintain it under laboratory conditions. To better understand this symbiosis, the genome of the S. velum endosymbiont was sequenced. Results: Relative to the genomes of obligate symbiotic bacteria, which commonly undergo erosion and reduction, the S. velum symbiont genome was large (2.7 Mb), GC-rich (51%), and contained a large number (78) of mobile genetic elements. Comparative genomics identified sets of genes specific to the chemosynthetic lifestyle and necessary to sustain the symbiosis. In addition, a number of inferred metabolic pathways and cellular processes, including heterotrophy, branched electron transport, and motility, suggested that besides the ability to function as an endosymbiont, the bacterium may have the capacity to live outside the host. Conclusions: The physiological dexterity indicated by the genome substantially improves our understanding of the genetic and metabolic capabilities of the S. velum symbiont and the breadth of niches the partners may inhabit during their lifecycle.

Original languageEnglish (US)
Article number924
JournalBMC Genomics
Volume15
Issue number1
DOIs
StatePublished - Oct 23 2014

Keywords

  • Calvin cycle
  • Chemosynthesis
  • H/Na -membrane cycles
  • Heterotrophy
  • Mobile genetic elements
  • Motility
  • Pyrophosphate-dependent phosphofructokinase
  • Respiratory flexibility
  • Sulfur oxidation
  • Symbiosis

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

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    Dmytrenko, O., Russell, S. L., Loo, W. T., Fontanez, K. M., Liao, L., Roeselers, G., Sharma, R., Stewart, F. J., Newton, I. L. G., Woyke, T., Wu, D., Lang, J. M., Eisen, J. A., & Cavanaugh, C. M. (2014). The genome of the intracellular bacterium of the coastal bivalve, Solemya velum: A blueprint for thriving in and out of symbiosis. BMC Genomics, 15(1), [924]. https://doi.org/10.1186/1471-2164-15-924