The genetic structure of Aedes aegypti populations is driven by boat traffic in the Peruvian Amazon

Sarah Anne J. Guagliardo, Yoosook Lee, Amanda A. Pierce, Jacklyn Wong, Yui Yin Chu, Amy C. Morrison, Helvio Astete, Berry Brosi, Gonzalo Vazquez-Prokopec, Thomas W. Scott, Uriel Kitron, Steven T. Stoddard

Research output: Contribution to journalArticle

Abstract

In the Americas, as in much of the rest of the world, the dengue virus vector Aedes aegypti is found in close association with human habitations, often leading to high population densities of mosquitoes in urban settings. In the Peruvian Amazon, this vector has been expanding to rural communities over the last 10-15 years, but to date, the population genetic structure of Ae. aegypti in this region has not been characterized. To investigate the relationship between Ae. aegypti gene flow and human transportation networks, we characterized mosquito population structure using a panel of 8 microsatellite markers and linked results to various potential mechanisms for long-distance dispersal. Adult and immature Ae. aegypti (>20 individuals per site) were collected from Iquitos city and from six neighboring riverine communities, i.e., Nauta, Indiana, Mazan, Barrio Florida, Tamshiaco, and Aucayo. FST statistics indicate significant, but low to moderate differentiation for the majority of study site pairs. Population structure of Ae. aegypti is not correlated with the geographic distance between towns, suggesting that human transportation networks provide a reasonable explanation for the high levels of population mixing. Our results indicate that Ae. aegypti gene flow among sub-populations is greatest between locations with heavy boat traffic, such as Iquitos-Tamshiaco and Iquitos-Indiana-Mazan, and lowest between locations with little or no boat/road traffic between them such as Barrio Florida-Iquitos. Bayesian clustering analysis showed ancestral admixture among three genetic clusters; no single cluster was exclusive to any site. Our results are consistent with the hypothesis that human transportation networks, particularly riverways, are responsible for the geographic spread of Ae. aegypti in the Peruvian Amazon. Our findings are applicable to other regions of the world characterized by networks of urban islands connected by fluvial transport routes.

Original languageEnglish (US)
Pages (from-to)e0007552
JournalPLoS neglected tropical diseases
Volume13
Issue number9
DOIs
StatePublished - Sep 1 2019

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Ships
Aedes
Genetic Structures
Gene Flow
Culicidae
Population
Dengue Virus
Bayes Theorem
Population Genetics
Rural Population
Population Density
Islands
Microsatellite Repeats
Cluster Analysis

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Guagliardo, S. A. J., Lee, Y., Pierce, A. A., Wong, J., Chu, Y. Y., Morrison, A. C., ... Stoddard, S. T. (2019). The genetic structure of Aedes aegypti populations is driven by boat traffic in the Peruvian Amazon. PLoS neglected tropical diseases, 13(9), e0007552. https://doi.org/10.1371/journal.pntd.0007552

The genetic structure of Aedes aegypti populations is driven by boat traffic in the Peruvian Amazon. / Guagliardo, Sarah Anne J.; Lee, Yoosook; Pierce, Amanda A.; Wong, Jacklyn; Chu, Yui Yin; Morrison, Amy C.; Astete, Helvio; Brosi, Berry; Vazquez-Prokopec, Gonzalo; Scott, Thomas W.; Kitron, Uriel; Stoddard, Steven T.

In: PLoS neglected tropical diseases, Vol. 13, No. 9, 01.09.2019, p. e0007552.

Research output: Contribution to journalArticle

Guagliardo, SAJ, Lee, Y, Pierce, AA, Wong, J, Chu, YY, Morrison, AC, Astete, H, Brosi, B, Vazquez-Prokopec, G, Scott, TW, Kitron, U & Stoddard, ST 2019, 'The genetic structure of Aedes aegypti populations is driven by boat traffic in the Peruvian Amazon', PLoS neglected tropical diseases, vol. 13, no. 9, pp. e0007552. https://doi.org/10.1371/journal.pntd.0007552
Guagliardo, Sarah Anne J. ; Lee, Yoosook ; Pierce, Amanda A. ; Wong, Jacklyn ; Chu, Yui Yin ; Morrison, Amy C. ; Astete, Helvio ; Brosi, Berry ; Vazquez-Prokopec, Gonzalo ; Scott, Thomas W. ; Kitron, Uriel ; Stoddard, Steven T. / The genetic structure of Aedes aegypti populations is driven by boat traffic in the Peruvian Amazon. In: PLoS neglected tropical diseases. 2019 ; Vol. 13, No. 9. pp. e0007552.
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