The generation of human long-term marrow cultures from marrow depleted of Ia (HLA-DR) positive cells

A. Keating, Jerry S Powell, M. Takahashi, J. W. Singer

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Successful long-term cultures were generated from marrow buffy-coat cells incubated with either of two monoclonal anti-Ia antibodies, 7.2 and HBIOa, and complement. The mean CFU-GM toxicity for 7.2 and HBIOa was 81% and 94% respectively, of the complement control. Cultures generated from marrows treated with 7.2 and complement produced between 30% and 163% of the CFU-GM (mean, 78.2 ± 52.4%) and 33% of the BFU-E produced by the complement control cultures. Long-term cultures from marrows treated with the more cytotoxic HBIOa antibody yielded 261% of the CFU-GM present at culture initiation. Our data suggest that the progenitor cells necessary for generation of long-term cultures from human marrow are Ia-negative and may represent less mature stem cells than those measurable by semisolid colony assays. Furthermore, marrow treated with cytotoxic anti-Ia antibodies may be suitable for use in autologous transplantation.

Original languageEnglish (US)
Pages (from-to)1159-1162
Number of pages4
JournalBlood
Volume64
Issue number6
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

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