The generation of human long-term marrow cultures from marrow depleted of Ia (HLA-DR) positive cells

A. Keating, Jerry S Powell, M. Takahashi, J. W. Singer

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Successful long-term cultures were generated from marrow buffy-coat cells incubated with either of two monoclonal anti-Ia antibodies, 7.2 and HBIOa, and complement. The mean CFU-GM toxicity for 7.2 and HBIOa was 81% and 94% respectively, of the complement control. Cultures generated from marrows treated with 7.2 and complement produced between 30% and 163% of the CFU-GM (mean, 78.2 ± 52.4%) and 33% of the BFU-E produced by the complement control cultures. Long-term cultures from marrows treated with the more cytotoxic HBIOa antibody yielded 261% of the CFU-GM present at culture initiation. Our data suggest that the progenitor cells necessary for generation of long-term cultures from human marrow are Ia-negative and may represent less mature stem cells than those measurable by semisolid colony assays. Furthermore, marrow treated with cytotoxic anti-Ia antibodies may be suitable for use in autologous transplantation.

Original languageEnglish (US)
Pages (from-to)1159-1162
Number of pages4
JournalBlood
Volume64
Issue number6
StatePublished - 1984
Externally publishedYes

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HLA-DR Antigens
Bone Marrow
Granulocyte-Macrophage Progenitor Cells
Cells
Antibodies
Anti-Idiotypic Antibodies
Stem cells
Cell culture
Stem Cells
Toxicity
Assays
Glycocalyx
Erythroid Precursor Cells
Autologous Transplantation
Monoclonal Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

The generation of human long-term marrow cultures from marrow depleted of Ia (HLA-DR) positive cells. / Keating, A.; Powell, Jerry S; Takahashi, M.; Singer, J. W.

In: Blood, Vol. 64, No. 6, 1984, p. 1159-1162.

Research output: Contribution to journalArticle

Keating, A, Powell, JS, Takahashi, M & Singer, JW 1984, 'The generation of human long-term marrow cultures from marrow depleted of Ia (HLA-DR) positive cells', Blood, vol. 64, no. 6, pp. 1159-1162.
Keating, A. ; Powell, Jerry S ; Takahashi, M. ; Singer, J. W. / The generation of human long-term marrow cultures from marrow depleted of Ia (HLA-DR) positive cells. In: Blood. 1984 ; Vol. 64, No. 6. pp. 1159-1162.
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