The gene encoding rat liver glycogen phosphorylase contains multiple polyadenylation signal sequences

Byron E. Froman, Robert C. Tait, Fredric A Gorin, Barbara A Horwitz, Judith S. Stern

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

RNA blot analysis of rat liver and adipose tissues detected two glycogen phosphorylase (GP)-encoding transcripts. The polymerase chain reaction was used to characterize the 3′-noncoding region of the gene (L-GP) encoding liver-GP (L-GP) from the lean Zucker rat (Fa/Fa). Three distinct classes of colinear cDNA clones were identified by nucleotide (nt) sequence analysis, demonstrating that the L-GP gene contains at least three functional polyadenylation sites. The predominant L-GP transcript was generated by polyadenylation 130 nt 3′ from the end of the coding region. A previously uncharacterized L-GP transcript is generated by polyadenylation at 346 nt 3′ of the first polyadenylation site. Polyadenylation site selection does not appear to be regulated in a tissue-specific fashion. The relative steady-state L-GP mRNA levels in the different types of adipose tissues were comparable to, or exceeded transcript levels in liver.

Original languageEnglish (US)
Pages (from-to)269-274
Number of pages6
JournalGene
Volume109
Issue number2
DOIs
StatePublished - Dec 30 1991

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Keywords

  • nucleotide sequence
  • polymerase chain reaction
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

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