The formation of cortical actin arrays in human trabecular meshwork cells in response to cytoskeletal disruption

Kaitlin C. Murphy, Joshua T. Morgan, Joshua Wood, Adeline Sadeli, Christopher J Murphy, Paul Russell

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The cytoskeleton of human trabecular meshwork (HTM) cells is known to be altered in glaucoma and has been hypothesized to reduce outflow facility through contracting the HTM tissue. Latrunculin B (Lat-B) and Rho-associated protein kinase (ROCK) inhibitors disrupt the actin cytoskeleton and are in clinical trials as glaucoma therapeutics. We have previously reported a transient increase in HTM cell stiffness peaking at 90. min after Lat-B treatment with a return to pretreatment values after 270. min. We hypothesize that changes in actin morphology correlate with alterations in cell stiffness induced by Lat-B but this is not a general consequence of other cytoskeletal disrupting agents such as Rho kinase inhibitors. We treated HTM cells with 2. μM Lat-B or 100. μM Y-27632 and allowed the cells to recover for 30-270. min. While examining actin morphology in Lat-B treated cells, we observed striking cortical actin arrays (CAAs). The percentage of CAA positive cells (CPCs) was time dependent and exceeded 30% at 90. min and decreased after 270. min. Y-27632 treated cells exhibited few CAAs and no changes in cell stiffness. Together, these data suggest that the increase in cell stiffness after Lat-B treatment is correlated with CAAs.

Original languageEnglish (US)
Pages (from-to)164-171
Number of pages8
JournalExperimental Cell Research
Volume328
Issue number1
DOIs
StatePublished - Oct 15 2014

Keywords

  • Actin
  • Cytoskeleton
  • Latrunculin B
  • Rho-associated protein kinase
  • Trabecular meshwork

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

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