Ensefalitli ve anemili koyunda Chaphamaparvovirüs'ün ilk keşfi

Translated title of the contribution: The first discovery of Chaphamaparvovirus in sheep with encephalitis and anemia

Eda Altan, Gilberto Sabino-Santos, Patricia Pesavento, Eric Delwart

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: Parvoviruses have been shown to exist in sheep since 1987. To this date there are only three reports pertaining to the existence parvoviruses in sheep. The first reported parvovirus study did not provide genomic information whereas the latter two belonged to the tetraparvovirus and copiparvovirus genera. This study focused on discovering the possible reasons of encephalitis and anemia in a dead sheep whose tissue samples were submitted to our laboratory. Methods: In the present study next-generation sequencing (NGS) was utilized. Nextera™ XT Sample Preparation Kit was used to generate a library for Illumina MiSeq using dual barcoding. An in-house pipeline was used for analyzing raw data generated from Miseq. Several software were used to create in housepipeline to trim sequences and de novo assembly. For the alignment genome and phylogenetic tree Geneious and MEGA X software were used. Results: A novel ovine chaphamaparvovirus and pestivirus D both were characterized simultaneously. Although chaphamaparvoviruses had had been reported in various animals, this is the first time they have been reported in sheep. PCR analyses confirmed the presence of chaphamaparvovirus in multiple tissues. The partial nonstructural protein (NS1) and the complete capsid proteins (VP1) protein sequences displayed the closest amino acid identity of 49% and 69%, respectively, to the proteins of a non-human primate chapparvovirus from Macaca fascicularis. Conclusion: This ovine parvovirus is the fourth parvovirus and the first chaphamaparvovirus reported in sheep. Both chaphamaparvovirus and pestivirus were shown to co-exist simultaneously in sheep. The role of this dual virus infection in the disease signs of this sheep remains to be determined. This study will shed light on future chaphamaparvovirus studies in sheep.

Translated title of the contributionThe first discovery of Chaphamaparvovirus in sheep with encephalitis and anemia
Original languageTurkish
Pages (from-to)343-350
Number of pages8
JournalTurk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology
Volume78
Issue number3
DOIs
StatePublished - 2021

Keywords

  • Chaphamaparvovirus
  • Chaphamaparvovirus
  • küçükbaş hayvan
  • Next-generation sequencing
  • ovine
  • Parvovirus
  • Parvovirus
  • Yeni nesil dizileme

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

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