The expression of tenascin-C with the AD1 variable repeat in embryonic tissues, cell lines and tumors in various vertebrate species

Laura B. Derr, Ruth Chiquet-Ehrismann, Regina F Gandour-Edwards, Jennnifer Spence, Richard P Tucker

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Tenascin-C is a modular glycoprotein composed of domains of amino acid repeats. All forms of tenascin-C have eight constant fibronectin type III repeats, but additional fibronectin type III repeat can be spliced into a variable domain found between the fifth and sixth constant repeats. Four extra repeats, named A, B, C and D, have been examined previously. Here, we have used in situ hybridization to determine the tissue origins of the novel AD1 and AD2 repeats. In the embryonic-day-10 chicken embryo, transcripts encoding the AD2 repeat are limited to the tips of lung bronchioles and the base of feather buds. In contrast the AD1 hybridization signal was widespread. Quantitative in situ hybridization reveals AD1-containing transcripts represent up to 85% of the total tenascin-C mRNA in some tissues (developing bone), and are undetectable in others (e.g. radial glia). Avian and human tumor cell lines were examined for the expression of the AD1 repeat using the reverse transcriptase polymerase chain reaction (RT-PCR). Transcripts encoding six different tenascin-C splice variants incorporating the AD1 repeat were found in the fibrosarcoma cell line, QT6. Many human tumor cells, including malignant melanoma and ductal breast carcinoma, were positive for AD1 tenascin-C expression. In addition, we found evidence of AD1 tenascin-C expression in samples of excised human tumors. Our results show that a novel variant may be a major part of the tenascin-C of the embryonic extracellular matrix, and may also be found in the stroma surrounding some human tumors.

Original languageEnglish (US)
Pages (from-to)71-82
Number of pages12
JournalDifferentiation
Volume62
Issue number2
DOIs
StatePublished - 1997

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Tenascin
Tumor Cell Line
Vertebrates
Fibronectins
In Situ Hybridization
Carcinoma, Ductal, Breast
Bronchioles
Neoplasms
Feathers
Fibrosarcoma
Reverse Transcriptase Polymerase Chain Reaction
Neuroglia
Extracellular Matrix
Chickens
Melanoma
Glycoproteins
Embryonic Structures
Amino Acids
Bone and Bones
Cell Line

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Developmental Biology
  • Cell Biology

Cite this

The expression of tenascin-C with the AD1 variable repeat in embryonic tissues, cell lines and tumors in various vertebrate species. / Derr, Laura B.; Chiquet-Ehrismann, Ruth; Gandour-Edwards, Regina F; Spence, Jennnifer; Tucker, Richard P.

In: Differentiation, Vol. 62, No. 2, 1997, p. 71-82.

Research output: Contribution to journalArticle

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