The expression of a NMDA receptor gene in guinea-pig myenteric plexus

D. L. Broussard, X. Li, D. B. Pritchett, D. Lynch, P. G C Bannermann, David E Pleasure

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Muscle tension studies of guinea-pig ileum longitudinal muscle-myenteric plexus preparations suggest that the N-methyl-D-aspartate (NMDA) receptor may be present in the enteric nervous system. Therefore, we investigated the expression of a gene for the NMDA receptor in guinea-pig taenia coli. The gene product was amplified using polymerase chain reaction (PCR) and its synthesis localized using in situ hybridization. A NMDA receptor PCR product from the myenteric plexus was demonstrated with nearly identical sequence characteristics to that in the brain. In situ hybridization studies identified myenteric neurons which express NMDA receptor messenger RNA. Demonstration of the genetic expression of the NMDA receptor supports a role for glutamate as a neurotransmitter in the enteric nervous system.

Original languageEnglish (US)
Pages (from-to)973-976
Number of pages4
Issue number8
StatePublished - 1994
Externally publishedYes


  • excitatory amino acid
  • gene expression
  • guinea-pig
  • myenteric plexus
  • N-methyl-D-aspartate receptor

ASJC Scopus subject areas

  • Neuroscience(all)


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