Abstract
Aim of the study: To investigate the expression of β-arrestins in fibroblast-like synoviocytes (FLS) from collagen-induced arthritis (CIA) rats and the effect of total glucosides of paeony (TGP). Materials and methods: TGP and glucosides of tripterygium wilfordii (GTW) were intragastriclly administrated to collagen-induced arthritis (CIA) rats after immunization. The secondary inflammatory reaction was evaluated by hind paw swelling, polyarthritis index and histopathological changes. Antibodies to type II collagen (CII) were determined by enzyme-linked immunosorbent assay (ELISA). Synoviocyte proliferations were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl (MTT) assay. The expression of β-arrestins in synoviocytes from CIA rats was measured by western blot. Results: The administration of TGP (25, 50, 100 mg/kg) depressed hind paw swelling and decreased the arthritis scores of CIA rats. TGP improved the pathologic manifestations of CIA. Serum anti-CII antibodies level increased significantly in CIA rats, while TGP had no effect on it. Fibroblast-like synoviocytes (FLS) proliferation was inhibited by TGP (50, 100 mg/kg). On d14, d28 after immunization, β-arrestins expression greatly up-regulated in synoviocytes from CIA rats and then returned to baseline levels on d42 after immunization. TGP (50, 100 mg/kg) significantly reduced the expression of β-arrestins. Conclusion: An inflammatory process in vivo induces an up-regulation of β-arrestins in synoviocytes from CIA rats while TGP can inhibit this change, which might be one of the important mechanisms for TGP to produce a marked therapeutic effect on RA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 511-516 |
| Number of pages | 6 |
| Journal | Journal of Ethnopharmacology |
| Volume | 133 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 27 2011 |
| Externally published | Yes |
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Keywords
- β-Arrestin
- Collagen-induced arthritis
- Desensitization
- Synoviocytes
- Total glucosides of paeony
ASJC Scopus subject areas
- Pharmacology
- Drug Discovery
Cite this
The expression change of β-arrestins in fibroblast-like synoviocytes from rats with collagen-induced arthritis and the effect of total glucosides of paeony. / Wang, Qingtong; Zhang, Ling Ling; Wu, Hua Xun; Wei, Wei.
In: Journal of Ethnopharmacology, Vol. 133, No. 2, 27.01.2011, p. 511-516.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The expression change of β-arrestins in fibroblast-like synoviocytes from rats with collagen-induced arthritis and the effect of total glucosides of paeony
AU - Wang, Qingtong
AU - Zhang, Ling Ling
AU - Wu, Hua Xun
AU - Wei, Wei
PY - 2011/1/27
Y1 - 2011/1/27
N2 - Aim of the study: To investigate the expression of β-arrestins in fibroblast-like synoviocytes (FLS) from collagen-induced arthritis (CIA) rats and the effect of total glucosides of paeony (TGP). Materials and methods: TGP and glucosides of tripterygium wilfordii (GTW) were intragastriclly administrated to collagen-induced arthritis (CIA) rats after immunization. The secondary inflammatory reaction was evaluated by hind paw swelling, polyarthritis index and histopathological changes. Antibodies to type II collagen (CII) were determined by enzyme-linked immunosorbent assay (ELISA). Synoviocyte proliferations were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl (MTT) assay. The expression of β-arrestins in synoviocytes from CIA rats was measured by western blot. Results: The administration of TGP (25, 50, 100 mg/kg) depressed hind paw swelling and decreased the arthritis scores of CIA rats. TGP improved the pathologic manifestations of CIA. Serum anti-CII antibodies level increased significantly in CIA rats, while TGP had no effect on it. Fibroblast-like synoviocytes (FLS) proliferation was inhibited by TGP (50, 100 mg/kg). On d14, d28 after immunization, β-arrestins expression greatly up-regulated in synoviocytes from CIA rats and then returned to baseline levels on d42 after immunization. TGP (50, 100 mg/kg) significantly reduced the expression of β-arrestins. Conclusion: An inflammatory process in vivo induces an up-regulation of β-arrestins in synoviocytes from CIA rats while TGP can inhibit this change, which might be one of the important mechanisms for TGP to produce a marked therapeutic effect on RA.
AB - Aim of the study: To investigate the expression of β-arrestins in fibroblast-like synoviocytes (FLS) from collagen-induced arthritis (CIA) rats and the effect of total glucosides of paeony (TGP). Materials and methods: TGP and glucosides of tripterygium wilfordii (GTW) were intragastriclly administrated to collagen-induced arthritis (CIA) rats after immunization. The secondary inflammatory reaction was evaluated by hind paw swelling, polyarthritis index and histopathological changes. Antibodies to type II collagen (CII) were determined by enzyme-linked immunosorbent assay (ELISA). Synoviocyte proliferations were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl (MTT) assay. The expression of β-arrestins in synoviocytes from CIA rats was measured by western blot. Results: The administration of TGP (25, 50, 100 mg/kg) depressed hind paw swelling and decreased the arthritis scores of CIA rats. TGP improved the pathologic manifestations of CIA. Serum anti-CII antibodies level increased significantly in CIA rats, while TGP had no effect on it. Fibroblast-like synoviocytes (FLS) proliferation was inhibited by TGP (50, 100 mg/kg). On d14, d28 after immunization, β-arrestins expression greatly up-regulated in synoviocytes from CIA rats and then returned to baseline levels on d42 after immunization. TGP (50, 100 mg/kg) significantly reduced the expression of β-arrestins. Conclusion: An inflammatory process in vivo induces an up-regulation of β-arrestins in synoviocytes from CIA rats while TGP can inhibit this change, which might be one of the important mechanisms for TGP to produce a marked therapeutic effect on RA.
KW - β-Arrestin
KW - Collagen-induced arthritis
KW - Desensitization
KW - Synoviocytes
KW - Total glucosides of paeony
UR - http://www.scopus.com/inward/record.url?scp=78651407340&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78651407340&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2010.10.022
DO - 10.1016/j.jep.2010.10.022
M3 - Article
C2 - 20965243
AN - SCOPUS:78651407340
VL - 133
SP - 511
EP - 516
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
SN - 0378-8741
IS - 2
ER -