The expression change of β-arrestins in fibroblast-like synoviocytes from rats with collagen-induced arthritis and the effect of total glucosides of paeony

Qingtong Wang, Ling Ling Zhang, Hua Xun Wu, Wei Wei

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Aim of the study: To investigate the expression of β-arrestins in fibroblast-like synoviocytes (FLS) from collagen-induced arthritis (CIA) rats and the effect of total glucosides of paeony (TGP). Materials and methods: TGP and glucosides of tripterygium wilfordii (GTW) were intragastriclly administrated to collagen-induced arthritis (CIA) rats after immunization. The secondary inflammatory reaction was evaluated by hind paw swelling, polyarthritis index and histopathological changes. Antibodies to type II collagen (CII) were determined by enzyme-linked immunosorbent assay (ELISA). Synoviocyte proliferations were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl (MTT) assay. The expression of β-arrestins in synoviocytes from CIA rats was measured by western blot. Results: The administration of TGP (25, 50, 100 mg/kg) depressed hind paw swelling and decreased the arthritis scores of CIA rats. TGP improved the pathologic manifestations of CIA. Serum anti-CII antibodies level increased significantly in CIA rats, while TGP had no effect on it. Fibroblast-like synoviocytes (FLS) proliferation was inhibited by TGP (50, 100 mg/kg). On d14, d28 after immunization, β-arrestins expression greatly up-regulated in synoviocytes from CIA rats and then returned to baseline levels on d42 after immunization. TGP (50, 100 mg/kg) significantly reduced the expression of β-arrestins. Conclusion: An inflammatory process in vivo induces an up-regulation of β-arrestins in synoviocytes from CIA rats while TGP can inhibit this change, which might be one of the important mechanisms for TGP to produce a marked therapeutic effect on RA.

Original languageEnglish (US)
Pages (from-to)511-516
Number of pages6
JournalJournal of Ethnopharmacology
Volume133
Issue number2
DOIs
StatePublished - Jan 27 2011
Externally publishedYes

Keywords

  • β-Arrestin
  • Collagen-induced arthritis
  • Desensitization
  • Synoviocytes
  • Total glucosides of paeony

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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