The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells

Takashi Kanaya, Koji Hase, Daisuke Takahashi, Shinji Fukuda, Katsuaki Hoshino, Izumi Sasaki, Hiroaki Hemmi, Kathryn A. Knoop, Nachiket Kumar, Mayuko Sato, Tatsuro Katsuno, Osamu Yokosuka, Kiminori Toyooka, Kumiko Nakai, Ayako Sakamoto, Yuuki Kitahara, Toshi Jinnohara, Stephen J Mcsorley, Tsuneyasu Kaisho, Ifor R. Williams & 1 others Hiroshi Ohno

Research output: Contribution to journalArticle

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Abstract

Intestinal microfold cells (M cells) are an enigmatic lineage of intestinal epithelial cells that initiate mucosal immune responses through the uptake and transcytosis of luminal antigens. The mechanisms of M-cell differentiation are poorly understood, as the rarity of these cells has hampered analysis. Exogenous administration of the cytokine RANKL can synchronously activate M-cell differentiation in mice. Here we show the Ets transcription factor Spi-B was induced early during M-cell differentiation. Absence of Spi-B silenced the expression of various M-cell markers and prevented the differentiation of M cells in mice. The activation of T cells via an oral route was substantially impaired in the intestine of Spi-B-deficient (Spib -/-) mice. Our study demonstrates that commitment to the intestinal M-cell lineage requires Spi-B as a candidate master regulator.

Original languageEnglish (US)
Pages (from-to)729-736
Number of pages8
JournalNature Immunology
Volume13
Issue number8
DOIs
StatePublished - Aug 2012

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Proto-Oncogene Proteins c-ets
Cell Differentiation
Cell Lineage
Transcytosis
Mucosal Immunity
Differentiation Antigens
Intestines
Epithelial Cells
Cytokines
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Kanaya, T., Hase, K., Takahashi, D., Fukuda, S., Hoshino, K., Sasaki, I., ... Ohno, H. (2012). The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells. Nature Immunology, 13(8), 729-736. https://doi.org/10.1038/ni.2352

The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells. / Kanaya, Takashi; Hase, Koji; Takahashi, Daisuke; Fukuda, Shinji; Hoshino, Katsuaki; Sasaki, Izumi; Hemmi, Hiroaki; Knoop, Kathryn A.; Kumar, Nachiket; Sato, Mayuko; Katsuno, Tatsuro; Yokosuka, Osamu; Toyooka, Kiminori; Nakai, Kumiko; Sakamoto, Ayako; Kitahara, Yuuki; Jinnohara, Toshi; Mcsorley, Stephen J; Kaisho, Tsuneyasu; Williams, Ifor R.; Ohno, Hiroshi.

In: Nature Immunology, Vol. 13, No. 8, 08.2012, p. 729-736.

Research output: Contribution to journalArticle

Kanaya, T, Hase, K, Takahashi, D, Fukuda, S, Hoshino, K, Sasaki, I, Hemmi, H, Knoop, KA, Kumar, N, Sato, M, Katsuno, T, Yokosuka, O, Toyooka, K, Nakai, K, Sakamoto, A, Kitahara, Y, Jinnohara, T, Mcsorley, SJ, Kaisho, T, Williams, IR & Ohno, H 2012, 'The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells', Nature Immunology, vol. 13, no. 8, pp. 729-736. https://doi.org/10.1038/ni.2352
Kanaya, Takashi ; Hase, Koji ; Takahashi, Daisuke ; Fukuda, Shinji ; Hoshino, Katsuaki ; Sasaki, Izumi ; Hemmi, Hiroaki ; Knoop, Kathryn A. ; Kumar, Nachiket ; Sato, Mayuko ; Katsuno, Tatsuro ; Yokosuka, Osamu ; Toyooka, Kiminori ; Nakai, Kumiko ; Sakamoto, Ayako ; Kitahara, Yuuki ; Jinnohara, Toshi ; Mcsorley, Stephen J ; Kaisho, Tsuneyasu ; Williams, Ifor R. ; Ohno, Hiroshi. / The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells. In: Nature Immunology. 2012 ; Vol. 13, No. 8. pp. 729-736.
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