The Escherichia coli homologue of yeast Rer2, a key enzyme of dolichol synthesis, is essential for carrier lipid formation in bacterial cell wall synthesis

Jun Ichi Kato, Shingo Fujisaki, Kenichi Nakajima, Yukinobu Nishimura, Miyuki Sato, Akihiko Nakano

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We found in the Escherichia coli genome sequence a homologue of RER2, a Saccharomyces cerevisiae gene required for proper localization of an endoplasmic reticulum protein, and designated it rth (RER2 homologue). The disruption of this gene was lethal for E. coli. To reveal its biological function, we isolated temperature-sensitive mutants of the rth gene. The mutant cells became swollen and burst at the nonpermissive temperature, indicating that their cell wall integrity was defective. Further analysis showed that the mutant cells were deficient in the activity of cis- prenyltransferase, namely, undecaprenyl diphosphate synthase, a key enzyme of the carrier lipid formation of peptidoglycan synthesis. The cellular level of undecaprenyl phosphate was in fact markedly decreased in the mutants. These results are consistent with the fact that the Rer2 homologue of Micrococcus luteus shows undecaprenyl diphosphate synthase activity (N. Shimizu, T. Koyama, and K. Ogura, J. Biol. Chem. 273:19476-19481, 1998) and demonstrate that E. coli Rth is indeed responsible for the maintenance of cell wall rigidity. Our work on the yeast rer2 mutants shows that they are defective in the activity of cis-prenyltransferase, namely, dehydrodolichyl diphosphate synthase, a key enzyme of dolichol synthesis. Taking these data together, we conclude that the RER2 gene family encodes cis-prenyltransferase, which plays an essential role in cell wall biosynthesis in bacteria and in dolichol synthesis in eukaryotic cells and has been well conserved during evolution.

Original languageEnglish (US)
Pages (from-to)2733-2738
Number of pages6
JournalJournal of Bacteriology
Volume181
Issue number9
StatePublished - May 1 1999
Externally publishedYes

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undecaprenyl pyrophosphate synthetase
Dolichol
Cell Wall
Yeasts
Escherichia coli
Lipids
Enzymes
Lethal Genes
Micrococcus luteus
Genes
Temperature
Peptidoglycan
Eukaryotic Cells
Sequence Homology
Endoplasmic Reticulum
Saccharomyces cerevisiae
Maintenance
Genome
Bacteria
cis-prenyl transferase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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The Escherichia coli homologue of yeast Rer2, a key enzyme of dolichol synthesis, is essential for carrier lipid formation in bacterial cell wall synthesis. / Kato, Jun Ichi; Fujisaki, Shingo; Nakajima, Kenichi; Nishimura, Yukinobu; Sato, Miyuki; Nakano, Akihiko.

In: Journal of Bacteriology, Vol. 181, No. 9, 01.05.1999, p. 2733-2738.

Research output: Contribution to journalArticle

Kato, Jun Ichi ; Fujisaki, Shingo ; Nakajima, Kenichi ; Nishimura, Yukinobu ; Sato, Miyuki ; Nakano, Akihiko. / The Escherichia coli homologue of yeast Rer2, a key enzyme of dolichol synthesis, is essential for carrier lipid formation in bacterial cell wall synthesis. In: Journal of Bacteriology. 1999 ; Vol. 181, No. 9. pp. 2733-2738.
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