The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes

Hsin Ya Yang, Roch Philippe Charles, Edith Hummler, Deborah L. Baines, Roslyn Rivkah Isseroff

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Cellular directional migration in an electric field (galvanotaxis) is one of the mechanisms guiding cell movement in embryogenesis and in skin epidermal repair. The epithelial sodium channel (ENaC), in addition to its function of regulating sodium transport in kidney, has recently been found to modulate cell locomotory speed. Here we tested whether ENaC has an additional function of mediating the directional migration of galvanotaxis in keratinocytes. Genetic depletion of ENaC completely blocks only galvanotaxis and does not decrease migration speed. Overexpression of ENaC is sufficient to drive galvanotaxis in otherwise unresponsive cells. Pharmacologic blockade or maintenance of the open state of ENaC also decreases or increases, respectively, galvanotaxis, suggesting that the channel open state is responsible for the response. Stable lamellipodial extensions formed at the cathodal sides of wild-type cells at the start of galvanotaxis; these were absent in the ENaC knockout keratinocytes, suggesting that ENaC mediates galvanotaxis by generating stable lamellipodia that steer cell migration. We provide evidence that ENaC is required for directional migration of keratinocytes in an electric field, supporting a role for ENaC in skin wound healing.

Original languageEnglish (US)
Pages (from-to)1942-1951
Number of pages10
JournalJournal of Cell Science
Volume126
Issue number9
DOIs
StatePublished - May 2013

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Epithelial Sodium Channels
Keratinocytes
Cell Movement
Skin
Pseudopodia
Taxis Response
Wound Healing
Embryonic Development
Sodium
Maintenance
Kidney

Keywords

  • Directional migration
  • ENaC
  • Keratinocyte
  • Lamellipodia

ASJC Scopus subject areas

  • Cell Biology

Cite this

The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes. / Yang, Hsin Ya; Charles, Roch Philippe; Hummler, Edith; Baines, Deborah L.; Isseroff, Roslyn Rivkah.

In: Journal of Cell Science, Vol. 126, No. 9, 05.2013, p. 1942-1951.

Research output: Contribution to journalArticle

Yang, Hsin Ya ; Charles, Roch Philippe ; Hummler, Edith ; Baines, Deborah L. ; Isseroff, Roslyn Rivkah. / The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes. In: Journal of Cell Science. 2013 ; Vol. 126, No. 9. pp. 1942-1951.
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