The effects of therapeutic concentrations of gentamicin, amikacin and hyaluronic acid on cultured bone marrow-derived equine mesenchymal stem cells

L. K. Bohannon, Sean D Owens, N. J. Walker, D. D. Carrade, Larry D Galuppo, Dori L Borjesson

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Reasons for performing study: Joint inflammation and septic arthritis are both potential complications of intra-articular injections of bone marrow-derived mesenchymal stem cells (BM-MSCs). Clinicians may prophylactically co-inject BM-MSCs admixed with either antimicrobials or hyaluronic acid; however, the effect of these agents on cultured BM-MSCs is unknown. Objective: To determine the effects of therapeutic levels of gentamicin, amikacin and hyaluronic acid on cultured equine BM-MSCs in vitro. Study design: In vitro experimental study. Methods: Equine BM-MSCs from 4 healthy mature horses were isolated. Cultured BM-MSCs from each donor were incubated with gentamicin (150mg), amikacin (250mg), hyaluronic acid (22mg) or 1% penicillin/streptomycin (control) under sterile conditions. Mesenchymal stem cells viability, proliferation, mediator secretion and culture media pH were measured. Results: Incubation of BM-MSCs with gentamicin resulted in >95% MSC death after 45min, and incubation of BM-MSCs with amikacin resulted in >95% MSC death after 2h. Incubation of BM-MSCs with hyaluronic acid or penicillin/streptomycin (control) for up to 6h resulted in sustained BM-MSC viability of 80% and >93%, respectively. All additives resulted in decreased media pH in the first minute; however, the pH then remained constant over the 6 h incubation period. No significant differences in BM-MSC proliferation or mediator secretion between the penicillin/streptomycin (control) and cells treated with hyaluronic acid were observed. Conclusion: Therapeutic concentrations of aminoglycoside antimicrobials are toxic to cultured equine BM-MSCs. The effects of hyaluronic acid on cultured MSC viability, proliferation and mediator secretion are minimal. Potential relevance: Based on these findings, the mixing of aminoglycoside antimicrobials and cultured equine BM-MSCs prior to therapeutic use is not recommended.

Original languageEnglish (US)
Pages (from-to)732-736
Number of pages5
JournalEquine Veterinary Journal
Volume45
Issue number6
DOIs
StatePublished - Nov 2013

Keywords

  • Antimicrobials
  • Bone marrow
  • Cell culture
  • Cytotoxicity
  • Horse
  • Mesenchymal stem cell

ASJC Scopus subject areas

  • Equine

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