The effects of some redox-active metals and reactive aldehydes on DNA-protein cross-links in vitro

Katherine L. Olin, Gary N. Cherr, Erik Rifkin, Carl L Keen

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

It has been suggested that the measurement of DNA-protein cross-links (DPCs) may be of value in the assessment of an individual's exposure to specific environmental insults. For a biomarker to be reliable, its results should be consistent and specific. In the present study, the precision and specificity of the K+-SDS precipitation assay as a measurement for DPCs was assessed. Chinese hamster ovary (CHO) and human fibroblast cells were exposed to a number of diverse oxidative insults, whose concentrations ranged from physiological to super-physiological levels. Only superphysiological concentrations of the insults induced the formation of DPCs. Formaldehyde, chromate, vanadate, acetaldehyde, and copper were round to be the greatest inducers of DPC formation, followed by manganese and iron. DPC induction was consistently higher in the CHO cells than in human fibroblast cells. While the K+ -SDS essay may be of value as an indicator of cumulative DNA damage, its value as a biomarker for specific environmental insults may be limited.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalToxicology
Volume110
Issue number1-3
DOIs
StatePublished - Jun 17 1996

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Keywords

  • Acetaldehyde
  • Chromium
  • Copper
  • DNA-protein cross-links
  • Formaldehyde
  • Iron
  • Manganese
  • Metals
  • Oxidative damage
  • Vanadium

ASJC Scopus subject areas

  • Toxicology

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