The effects of dicofol on induction of hepatic microsomal metabolism in rats

Bruce A. Narloch, Michael P. Lawton, David E. Moody, Bruce D. Hammock, Lee R. Shull

Research output: Contribution to journalArticle

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Abstract

In a comparative study, the induction effects of dicofol, technical Kelthane, and DDT on hepatic microsomal and cytosolic enzyme activities in rats were compared with those effects produced by phenobarbital (PhB) and β-naphthoflavone (BNF). Male rats (ca. 250 g) were injected (ip) for 4 consecutive days with 1.0 ml of vehicle containing either dicofol (1.5, 15.0, 29.5, or 59.0 mM, Kelthane (dicofol content equal to 29.5 or 59.0 mM), DDT (59.0 mM), or BNF (36.7 mM). Liver weights, microsomal protein, and cytochrome P-450 concentrations and microsomal and cytosolic enzyme specific activities were measured. Dicofol produced dose-related increases in all of the parameters measured except liver weight and cytosolic epoxide hydrolase activity. At a concentration of 59.0 mM, dicofol increased the concentrations of microsomal protein (1.7-fold) and cytochrome P-450 (2.9-fold), and the specific activities of cytochrome c reductase (1.6-fold), ethoxycoumarin O-deethylase (2.3-fold), aminopyrine N-demethylase (3.0-fold), microsomal epoxide hydrolase (2.6-fold), and glutathione S-transferase (2.9-fold). The induction potency of dicofol was equivalent to Kelthane, DDT, and PhB at equimolar (59.0 mM) concentrations of chemical.

Original languageEnglish (US)
Pages (from-to)362-370
Number of pages9
JournalPesticide Biochemistry and Physiology
Volume28
Issue number3
DOIs
StatePublished - 1987

Fingerprint

Dicofol
dicofol
Metabolism
Rats
liver
metabolism
Liver
rats
DDT
DDT (pesticide)
epoxide hydrolase
Epoxide Hydrolases
phenobarbital
Phenobarbital
cytochrome P-450
Cytochrome P-450 Enzyme System
Aminopyrine N-Demethylase
7-Alkoxycoumarin O-Dealkylase
Cytochrome Reductases
Weights and Measures

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Biochemistry
  • Physiology

Cite this

The effects of dicofol on induction of hepatic microsomal metabolism in rats. / Narloch, Bruce A.; Lawton, Michael P.; Moody, David E.; Hammock, Bruce D.; Shull, Lee R.

In: Pesticide Biochemistry and Physiology, Vol. 28, No. 3, 1987, p. 362-370.

Research output: Contribution to journalArticle

Narloch, Bruce A. ; Lawton, Michael P. ; Moody, David E. ; Hammock, Bruce D. ; Shull, Lee R. / The effects of dicofol on induction of hepatic microsomal metabolism in rats. In: Pesticide Biochemistry and Physiology. 1987 ; Vol. 28, No. 3. pp. 362-370.
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