Abstract
Alternative sources of lipoproteins in semen extenders could replace animal by-products. We hypothesized that: (1) post-thaw semen parameters and fertility would not be different in coconut water (CW)–treated samples compared with egg yolk (EY)–treated samples and (2) the use of an oxygen scavenger (Oxyrase) would improve post-thaw sperm motility and membrane integrity and decrease lipid peroxidation. Experiment 1: three ejaculates each from five stallions were split into four treatments: EY, CW, egg yolk with Oxyrase, and coconut water with Oxyrase. Computer-assisted sperm analysis measured progressive and total motility, velocity, and linearity. Membrane integrity, apoptosis, and lipid peroxidation were evaluated using propidium iodide, annexin, and BODIPY fluorescent probes, respectively. Samples were cryopreserved, stored in liquid nitrogen, and then thawed to 37°C and analyzed again. Experiment 2: one ejaculate was divided into two aliquots and cryopreserved using either CW or EY. In a crossover design, 12 mares were bred on two consecutive cycles with either EY or CW. Pregnancy evaluations were at 14-day gestation. No differences were detected in sperm parameters between CW and EY (P >.05). Oxyrase did not improve sperm motility parameters in post-thaw samples, nor did it show protective effects for viability or against membrane damage (P >.05). More mares became pregnant using CW than EY (11/12 vs. 6/12, respectively; P =.013). Use of CW is a viable alternative to animal-based products in the cryopreservation of stallion semen.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 51-57 |
| Number of pages | 7 |
| Journal | Journal of Equine Veterinary Science |
| Volume | 58 |
| DOIs | |
| State | Published - Nov 1 2017 |
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Keywords
- Coconut water
- Egg yolk
- Oxyrase
- Sperm cryopreservation
- Stallion sperm
ASJC Scopus subject areas
- Equine
Cite this
The Effects of an Oxygen Scavenger and Coconut Water on Equine Sperm Cryopreservation. / London, Kelly T.; Christensen, Bruce W; Scott, Camilla J.; Klooster, Katie; Kass, Philip H; Dujovne, Ghislaine; Meyers, Stuart A.
In: Journal of Equine Veterinary Science, Vol. 58, 01.11.2017, p. 51-57.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Effects of an Oxygen Scavenger and Coconut Water on Equine Sperm Cryopreservation
AU - London, Kelly T.
AU - Christensen, Bruce W
AU - Scott, Camilla J.
AU - Klooster, Katie
AU - Kass, Philip H
AU - Dujovne, Ghislaine
AU - Meyers, Stuart A
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Alternative sources of lipoproteins in semen extenders could replace animal by-products. We hypothesized that: (1) post-thaw semen parameters and fertility would not be different in coconut water (CW)–treated samples compared with egg yolk (EY)–treated samples and (2) the use of an oxygen scavenger (Oxyrase) would improve post-thaw sperm motility and membrane integrity and decrease lipid peroxidation. Experiment 1: three ejaculates each from five stallions were split into four treatments: EY, CW, egg yolk with Oxyrase, and coconut water with Oxyrase. Computer-assisted sperm analysis measured progressive and total motility, velocity, and linearity. Membrane integrity, apoptosis, and lipid peroxidation were evaluated using propidium iodide, annexin, and BODIPY fluorescent probes, respectively. Samples were cryopreserved, stored in liquid nitrogen, and then thawed to 37°C and analyzed again. Experiment 2: one ejaculate was divided into two aliquots and cryopreserved using either CW or EY. In a crossover design, 12 mares were bred on two consecutive cycles with either EY or CW. Pregnancy evaluations were at 14-day gestation. No differences were detected in sperm parameters between CW and EY (P >.05). Oxyrase did not improve sperm motility parameters in post-thaw samples, nor did it show protective effects for viability or against membrane damage (P >.05). More mares became pregnant using CW than EY (11/12 vs. 6/12, respectively; P =.013). Use of CW is a viable alternative to animal-based products in the cryopreservation of stallion semen.
AB - Alternative sources of lipoproteins in semen extenders could replace animal by-products. We hypothesized that: (1) post-thaw semen parameters and fertility would not be different in coconut water (CW)–treated samples compared with egg yolk (EY)–treated samples and (2) the use of an oxygen scavenger (Oxyrase) would improve post-thaw sperm motility and membrane integrity and decrease lipid peroxidation. Experiment 1: three ejaculates each from five stallions were split into four treatments: EY, CW, egg yolk with Oxyrase, and coconut water with Oxyrase. Computer-assisted sperm analysis measured progressive and total motility, velocity, and linearity. Membrane integrity, apoptosis, and lipid peroxidation were evaluated using propidium iodide, annexin, and BODIPY fluorescent probes, respectively. Samples were cryopreserved, stored in liquid nitrogen, and then thawed to 37°C and analyzed again. Experiment 2: one ejaculate was divided into two aliquots and cryopreserved using either CW or EY. In a crossover design, 12 mares were bred on two consecutive cycles with either EY or CW. Pregnancy evaluations were at 14-day gestation. No differences were detected in sperm parameters between CW and EY (P >.05). Oxyrase did not improve sperm motility parameters in post-thaw samples, nor did it show protective effects for viability or against membrane damage (P >.05). More mares became pregnant using CW than EY (11/12 vs. 6/12, respectively; P =.013). Use of CW is a viable alternative to animal-based products in the cryopreservation of stallion semen.
KW - Coconut water
KW - Egg yolk
KW - Oxyrase
KW - Sperm cryopreservation
KW - Stallion sperm
UR - http://www.scopus.com/inward/record.url?scp=85028704949&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85028704949&partnerID=8YFLogxK
U2 - 10.1016/j.jevs.2017.08.014
DO - 10.1016/j.jevs.2017.08.014
M3 - Article
AN - SCOPUS:85028704949
VL - 58
SP - 51
EP - 57
JO - Journal of Equine Veterinary Science
JF - Journal of Equine Veterinary Science
SN - 0737-0806
ER -