The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri

Wolfram M. Brück, Shannon L. Kelleher, Glenn R. Gibson, Gitte Graverholt, Bo L. Lönnerdal

Research output: Contribution to journalArticle

33 Scopus citations


Two milk components, α-lactalbumin (α-La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. 3[H] thymidine-labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo-2 cultures and their association with CaCo-2 cells was assessed. Undigested, pepsin-digested and pepsin- and pancreatin-digested α-lactalbumin and glycomacropeptide inhibited association. Thus, milk supplemented with α-lactalbumin and glycomacropeptide might be effective in inhibiting associations of the pathogens EPEC, Salmonella typhimurium, and Shigella flexneri to intestinal cells.

Original languageEnglish (US)
Pages (from-to)158-162
Number of pages5
JournalFEMS Microbiology Letters
Issue number1
StatePublished - Jun 2006



  • α-lactalbumin
  • Enteropathogenic Escherichia coli
  • Glycomacropeptide
  • Milk proteins
  • Salmonella spp.
  • Shigella spp.

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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