The effect of synbiotics Bifidobacterium infantis and milk oligosaccharides on shaping gut microbiota community structure and NASH treatment

Prasant Kumar Jena, Lili Sheng, Nidhi Nagar, Chao Wu, Daniela Barile, David A. Mills, Yu-Jui Yvonne Wan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Probiotic Bifidobacterium longum subspecies infantis (Bifidobacterium infantis) consumes human milk oligosaccharides (MO) and protects intestinal permeability thereby having anti-inflammatory effects (Underwood et al., 2015; Bode, 2006; Asakuma et al., 2011) [1–3]. Via the gut-liver axis, gut barrier disruption and dysbiosis lead to hepatic inflammation (Sheng et al., 2017; Jena et al., 2017) [4,5,6]. Our published data revealed that butyrate, as well as synbiotics of B. infantis in combination with MO, had protective effects against cancer-prone non-alcoholic steatohepatitis (NASH) mouse models, i.e., Western diet (WD)-fed bile acid receptor FXR (farnesoid x receptor) knockout (KO) mice (Jena et al., 2018) [6,7]. In addition, MO was particularly effective in increasing the blooming of butyrate-generating bacteria (Jena et al., 2018) [7]. In the present study, we further showed that the reduced ileal short chain fatty acid (SCFA) signaling found in WD-fed FXR KO mice could be reversed by B. infantis and/or MO treatment. Moreover, ileal mRNA levels of SCFA receptors i.e. Gpr41 (Ffar3), Gpr109 (Hcar2), and Gpr43 (Ffar2) were increased in B. infantis and/or MO-treated mice suggesting increased SCFA signaling (Fig. 1). Further, nuclear magnetic resonance (NMR) data revealed that MO and B. Infantis plus MO increased intestinal acetate, propionate, butyrate, and valerate levels (Fig. 2). In addition, B. infantis and/or MO reduced the abundance of genus Bilophila and the relative copy number of bacterial genes including dissimilatory sulfite reductase (dsrA) and methyl coenzyme M reductase A (mcrA), which were all increased in cancer-prone FXR KO mice (Fig. 3).

Original languageEnglish (US)
Pages (from-to)1025-1029
Number of pages5
JournalData in Brief
Volume19
DOIs
StatePublished - Aug 1 2018

Fingerprint

Synbiotics
Fatty Liver
Oligosaccharides
Milk
Volatile Fatty Acids
Butyrates
Knockout Mice
Bilophila
Hydrogensulfite Reductase
Dysbiosis
Valerates
Bacterial Genes
Gastrointestinal Microbiome
Bifidobacterium longum subspecies infantis
Liver
Propionates
Probiotics
Human Milk
Bile Acids and Salts
Permeability

Keywords

  • Inflammation
  • Probiotics
  • Short chain fatty acid
  • Western diet

ASJC Scopus subject areas

  • General

Cite this

The effect of synbiotics Bifidobacterium infantis and milk oligosaccharides on shaping gut microbiota community structure and NASH treatment. / Jena, Prasant Kumar; Sheng, Lili; Nagar, Nidhi; Wu, Chao; Barile, Daniela; Mills, David A.; Wan, Yu-Jui Yvonne.

In: Data in Brief, Vol. 19, 01.08.2018, p. 1025-1029.

Research output: Contribution to journalArticle

Jena, Prasant Kumar ; Sheng, Lili ; Nagar, Nidhi ; Wu, Chao ; Barile, Daniela ; Mills, David A. ; Wan, Yu-Jui Yvonne. / The effect of synbiotics Bifidobacterium infantis and milk oligosaccharides on shaping gut microbiota community structure and NASH treatment. In: Data in Brief. 2018 ; Vol. 19. pp. 1025-1029.
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AU - Mills, David A.

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