The effect of miRNA-122 in regulating fat deposition in a cell line model

Yu Qiang Nie, Jie Cao, Yong Jian Zhou, Xia Liang, Yan Lei Du, Yu-Jui Yvonne Wan, Yu Yuan Li

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Accumulating evidence supports the role of miR-122 in fatty liver disease. We investigated miR-122 expression in a steatotic hepatocyte model, the effect of miR-122 over-expression and inhibition in the pathogenesis. Human hepatic cell line L02 was induced with oleic acid to establish the steatotic hepatocyte model. Intracellular lipid content was observed with laser scanning confocal microscope (LSCM), and triglyceride content was determined with kits. Total RNA was extracted and reversely transcribed into cDNA. miR-122 expression was measured using qRT-PCR. Subsequently, miR-122 mimic and miR-122 inhibitor were transfected into steatotic hepatocytes to observe their effect on intracellular lipid content. The lipid fluorescence intensity and triglyceride content within the steatotic hepatocytes were significantly higher than those in normal control (860.01 ± 26.52 vs. 257.77 ± 29.69 and 3.47 ± 0.12 vs. 1.85 ± 0.02 at 24 h) (P < 0.01). miR-122 expression in steatotic hepatocytes was down-regulated compared with that in control (2-ΔCt value: 0.0286 ± 0.0078 vs. 0.0075 ± 0.0012) (P < 0.01). After transfection, miR-122 expression (2-ΔCt value) in the miR-122 mimic group increased 2.96-fold compared with that in control, and its lipid fluorescence intensity was significantly lower than that in control (790.92 ± 46.72 vs. 1,022.16 ± 49.66) (P < 0.01). Nevertheless, miR-122 expression decreased 3.45-fold in the miR-122 inhibitor group compared with that in control, and its fluorescence intensity was significantly higher than that in control (1,386.49 ± 40.34 vs 1,022.16 ± 49.66)(P < 0.01). We concluded that miR-122 was down-regulated in steatotic hepatocytes model. The pathogenesis of hepatocyte steatosis was enhanced by miR-122 mimic and reduced with miR-122 inhibitor.

Original languageEnglish (US)
Pages (from-to)839-846
Number of pages8
JournalJournal of Cellular Biochemistry
Volume115
Issue number5
DOIs
StatePublished - 2014

Fingerprint

MicroRNAs
Hepatocytes
Fats
Cells
Cell Line
Lipids
Fluorescence
Triglycerides
Fatty Liver
Oleic Acid
Liver
Transfection
Liver Diseases
Lasers
Microscopes
Complementary DNA
RNA
Scanning
Polymerase Chain Reaction

Keywords

  • FATTY LIVER DISEASE
  • HEPATOCYTE
  • miR-122
  • STEATOSIS

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The effect of miRNA-122 in regulating fat deposition in a cell line model. / Nie, Yu Qiang; Cao, Jie; Zhou, Yong Jian; Liang, Xia; Du, Yan Lei; Wan, Yu-Jui Yvonne; Li, Yu Yuan.

In: Journal of Cellular Biochemistry, Vol. 115, No. 5, 2014, p. 839-846.

Research output: Contribution to journalArticle

Nie, Yu Qiang ; Cao, Jie ; Zhou, Yong Jian ; Liang, Xia ; Du, Yan Lei ; Wan, Yu-Jui Yvonne ; Li, Yu Yuan. / The effect of miRNA-122 in regulating fat deposition in a cell line model. In: Journal of Cellular Biochemistry. 2014 ; Vol. 115, No. 5. pp. 839-846.
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AU - Wan, Yu-Jui Yvonne

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AB - Accumulating evidence supports the role of miR-122 in fatty liver disease. We investigated miR-122 expression in a steatotic hepatocyte model, the effect of miR-122 over-expression and inhibition in the pathogenesis. Human hepatic cell line L02 was induced with oleic acid to establish the steatotic hepatocyte model. Intracellular lipid content was observed with laser scanning confocal microscope (LSCM), and triglyceride content was determined with kits. Total RNA was extracted and reversely transcribed into cDNA. miR-122 expression was measured using qRT-PCR. Subsequently, miR-122 mimic and miR-122 inhibitor were transfected into steatotic hepatocytes to observe their effect on intracellular lipid content. The lipid fluorescence intensity and triglyceride content within the steatotic hepatocytes were significantly higher than those in normal control (860.01 ± 26.52 vs. 257.77 ± 29.69 and 3.47 ± 0.12 vs. 1.85 ± 0.02 at 24 h) (P < 0.01). miR-122 expression in steatotic hepatocytes was down-regulated compared with that in control (2-ΔCt value: 0.0286 ± 0.0078 vs. 0.0075 ± 0.0012) (P < 0.01). After transfection, miR-122 expression (2-ΔCt value) in the miR-122 mimic group increased 2.96-fold compared with that in control, and its lipid fluorescence intensity was significantly lower than that in control (790.92 ± 46.72 vs. 1,022.16 ± 49.66) (P < 0.01). Nevertheless, miR-122 expression decreased 3.45-fold in the miR-122 inhibitor group compared with that in control, and its fluorescence intensity was significantly higher than that in control (1,386.49 ± 40.34 vs 1,022.16 ± 49.66)(P < 0.01). We concluded that miR-122 was down-regulated in steatotic hepatocytes model. The pathogenesis of hepatocyte steatosis was enhanced by miR-122 mimic and reduced with miR-122 inhibitor.

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