Abstract
The objective of this study was to determine the effectiveness of oxygen removal in minimizing oxidative stress to improve fertile longevity of equine spermatozoa. Stallion ejaculates (n = 6) were treated with increasing doses of the oxygen scavenger Oxyrase (0.6 U/mL, 1.2 U/mL, 2.4 U/mL, and 5.0 U/mL). Samples were cryopreserved, thawed, and analyzed for motility over 24 hours at ambient temperature. These data suggest a role of excessive oxygen in the reduced motility of cryopreserved samples as 2.4 U/mL Oxyrase minimized the loss of post-thaw motility over the other doses and control treatments. Oxygen, a key component of reactive oxygen species generation, is directly involved in oxidative damage, and its removal may trigger metabolic changes that are more suited for post-thaw motility maintenance. Removal of oxygen from semen before cryopreservation may promote increased post-thaw fertility and may have the potential to overcome stallion variability in cryosurvival.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 44-51 |
| Number of pages | 8 |
| Journal | Journal of Equine Veterinary Science |
| Volume | 42 |
| DOIs | |
| State | Published - Jul 1 2016 |
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Keywords
- Cryopreservation
- Oxidative stress
- Oxyrase
- Sperm
- Stallion
ASJC Scopus subject areas
- Equine
Cite this
The Effect of Low Oxygen During the Early Phases of Sperm Freezing in Stallions With Low Progressive Motility : Can We Improve Post-Thaw Motility of Stallion Sperm? / Darr, Christa; Martorana, Kelly; Scanlan, Tawny; Meyers, Stuart A.
In: Journal of Equine Veterinary Science, Vol. 42, 01.07.2016, p. 44-51.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Effect of Low Oxygen During the Early Phases of Sperm Freezing in Stallions With Low Progressive Motility
T2 - Can We Improve Post-Thaw Motility of Stallion Sperm?
AU - Darr, Christa
AU - Martorana, Kelly
AU - Scanlan, Tawny
AU - Meyers, Stuart A
PY - 2016/7/1
Y1 - 2016/7/1
N2 - The objective of this study was to determine the effectiveness of oxygen removal in minimizing oxidative stress to improve fertile longevity of equine spermatozoa. Stallion ejaculates (n = 6) were treated with increasing doses of the oxygen scavenger Oxyrase (0.6 U/mL, 1.2 U/mL, 2.4 U/mL, and 5.0 U/mL). Samples were cryopreserved, thawed, and analyzed for motility over 24 hours at ambient temperature. These data suggest a role of excessive oxygen in the reduced motility of cryopreserved samples as 2.4 U/mL Oxyrase minimized the loss of post-thaw motility over the other doses and control treatments. Oxygen, a key component of reactive oxygen species generation, is directly involved in oxidative damage, and its removal may trigger metabolic changes that are more suited for post-thaw motility maintenance. Removal of oxygen from semen before cryopreservation may promote increased post-thaw fertility and may have the potential to overcome stallion variability in cryosurvival.
AB - The objective of this study was to determine the effectiveness of oxygen removal in minimizing oxidative stress to improve fertile longevity of equine spermatozoa. Stallion ejaculates (n = 6) were treated with increasing doses of the oxygen scavenger Oxyrase (0.6 U/mL, 1.2 U/mL, 2.4 U/mL, and 5.0 U/mL). Samples were cryopreserved, thawed, and analyzed for motility over 24 hours at ambient temperature. These data suggest a role of excessive oxygen in the reduced motility of cryopreserved samples as 2.4 U/mL Oxyrase minimized the loss of post-thaw motility over the other doses and control treatments. Oxygen, a key component of reactive oxygen species generation, is directly involved in oxidative damage, and its removal may trigger metabolic changes that are more suited for post-thaw motility maintenance. Removal of oxygen from semen before cryopreservation may promote increased post-thaw fertility and may have the potential to overcome stallion variability in cryosurvival.
KW - Cryopreservation
KW - Oxidative stress
KW - Oxyrase
KW - Sperm
KW - Stallion
UR - http://www.scopus.com/inward/record.url?scp=84964413024&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84964413024&partnerID=8YFLogxK
U2 - 10.1016/j.jevs.2016.03.022
DO - 10.1016/j.jevs.2016.03.022
M3 - Article
AN - SCOPUS:84964413024
VL - 42
SP - 44
EP - 51
JO - Journal of Equine Veterinary Science
JF - Journal of Equine Veterinary Science
SN - 0737-0806
ER -