The Effect of Low Oxygen During the Early Phases of Sperm Freezing in Stallions With Low Progressive Motility: Can We Improve Post-Thaw Motility of Stallion Sperm?

Christa Darr, Kelly Martorana, Tawny Scanlan, Stuart A Meyers

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The objective of this study was to determine the effectiveness of oxygen removal in minimizing oxidative stress to improve fertile longevity of equine spermatozoa. Stallion ejaculates (n = 6) were treated with increasing doses of the oxygen scavenger Oxyrase (0.6 U/mL, 1.2 U/mL, 2.4 U/mL, and 5.0 U/mL). Samples were cryopreserved, thawed, and analyzed for motility over 24 hours at ambient temperature. These data suggest a role of excessive oxygen in the reduced motility of cryopreserved samples as 2.4 U/mL Oxyrase minimized the loss of post-thaw motility over the other doses and control treatments. Oxygen, a key component of reactive oxygen species generation, is directly involved in oxidative damage, and its removal may trigger metabolic changes that are more suited for post-thaw motility maintenance. Removal of oxygen from semen before cryopreservation may promote increased post-thaw fertility and may have the potential to overcome stallion variability in cryosurvival.

Original languageEnglish (US)
Pages (from-to)44-51
Number of pages8
JournalJournal of Equine Veterinary Science
Volume42
DOIs
StatePublished - Jul 1 2016

Fingerprint

Sperm Motility
stallions
Freezing
Spermatozoa
freezing
spermatozoa
Oxygen
oxygen
overdose
free radical scavengers
cryopreservation
Cryopreservation
semen
reactive oxygen species
ambient temperature
Semen
oxidative stress
Horses
Fertility
Reactive Oxygen Species

Keywords

  • Cryopreservation
  • Oxidative stress
  • Oxyrase
  • Sperm
  • Stallion

ASJC Scopus subject areas

  • Equine

Cite this

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title = "The Effect of Low Oxygen During the Early Phases of Sperm Freezing in Stallions With Low Progressive Motility: Can We Improve Post-Thaw Motility of Stallion Sperm?",
abstract = "The objective of this study was to determine the effectiveness of oxygen removal in minimizing oxidative stress to improve fertile longevity of equine spermatozoa. Stallion ejaculates (n = 6) were treated with increasing doses of the oxygen scavenger Oxyrase (0.6 U/mL, 1.2 U/mL, 2.4 U/mL, and 5.0 U/mL). Samples were cryopreserved, thawed, and analyzed for motility over 24 hours at ambient temperature. These data suggest a role of excessive oxygen in the reduced motility of cryopreserved samples as 2.4 U/mL Oxyrase minimized the loss of post-thaw motility over the other doses and control treatments. Oxygen, a key component of reactive oxygen species generation, is directly involved in oxidative damage, and its removal may trigger metabolic changes that are more suited for post-thaw motility maintenance. Removal of oxygen from semen before cryopreservation may promote increased post-thaw fertility and may have the potential to overcome stallion variability in cryosurvival.",
keywords = "Cryopreservation, Oxidative stress, Oxyrase, Sperm, Stallion",
author = "Christa Darr and Kelly Martorana and Tawny Scanlan and Meyers, {Stuart A}",
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T2 - Can We Improve Post-Thaw Motility of Stallion Sperm?

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AU - Martorana, Kelly

AU - Scanlan, Tawny

AU - Meyers, Stuart A

PY - 2016/7/1

Y1 - 2016/7/1

N2 - The objective of this study was to determine the effectiveness of oxygen removal in minimizing oxidative stress to improve fertile longevity of equine spermatozoa. Stallion ejaculates (n = 6) were treated with increasing doses of the oxygen scavenger Oxyrase (0.6 U/mL, 1.2 U/mL, 2.4 U/mL, and 5.0 U/mL). Samples were cryopreserved, thawed, and analyzed for motility over 24 hours at ambient temperature. These data suggest a role of excessive oxygen in the reduced motility of cryopreserved samples as 2.4 U/mL Oxyrase minimized the loss of post-thaw motility over the other doses and control treatments. Oxygen, a key component of reactive oxygen species generation, is directly involved in oxidative damage, and its removal may trigger metabolic changes that are more suited for post-thaw motility maintenance. Removal of oxygen from semen before cryopreservation may promote increased post-thaw fertility and may have the potential to overcome stallion variability in cryosurvival.

AB - The objective of this study was to determine the effectiveness of oxygen removal in minimizing oxidative stress to improve fertile longevity of equine spermatozoa. Stallion ejaculates (n = 6) were treated with increasing doses of the oxygen scavenger Oxyrase (0.6 U/mL, 1.2 U/mL, 2.4 U/mL, and 5.0 U/mL). Samples were cryopreserved, thawed, and analyzed for motility over 24 hours at ambient temperature. These data suggest a role of excessive oxygen in the reduced motility of cryopreserved samples as 2.4 U/mL Oxyrase minimized the loss of post-thaw motility over the other doses and control treatments. Oxygen, a key component of reactive oxygen species generation, is directly involved in oxidative damage, and its removal may trigger metabolic changes that are more suited for post-thaw motility maintenance. Removal of oxygen from semen before cryopreservation may promote increased post-thaw fertility and may have the potential to overcome stallion variability in cryosurvival.

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