The effect of long-term hormonal treatment on voiding patterns during filling cystometry and on urethral histology in a postpartum, ovariectomized female rat

Benjamin N. Breyer, Guifang Wang, Guiting Lin, Alan W. Shindel, Rong Yang, Ching Shwun Lin, Tom F. Lue

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

OBJECTIVE To study whether long-term treatment with oestrogen (E 2), selective E2 receptor modulators (SERMs), or growth hormone (GH) can prevent the development of abnormal voiding patterns during filling cystometry (CMG) in a postpartum, ovariectomized (Ovx) female rat. MATERIALS AND METHODS Immediately after spontaneous delivery, 60 primiparous Sprague-Dawley rats were randomly divided into six equal groups. One group served as uninjured sham controls and five groups underwent intravaginal balloon dilatation. On day seven, previously dilated rats underwent bilateral Ovx and implantation of a subcutaneous hormone-delivery pump. The five treatment groups received normal saline (control), E2, raloxifene, levormeloxifene, or GH for 7 weeks. Conscious CMG was performed 7 weeks after Ovx. Urethral sphincter tissue was harvested for elastin immunohistochemistry and real-time polymerase chain reaction of α1A-adrenoceptor mRNA. RESULTS No abnormal voiding patterns were detected in the group treated with GH. The E 2, raloxifene and levormeloxifene groups had greater detrusor overactivity and urethral relaxation incontinence than control rats. The raloxifene group had a significantly lower baseline bladder pressure and opening pressure. GH-treated rats had higher elastin content in the urethra. Urethral α1A-adrenoceptor mRNA concentration was significantly lower in the SERM-treated rats compared with controls. CONCLUSIONS GH prevents the development of abnormal voiding patterns during filling CMG in a rat model of parturition-induced incontinence; E2 and SERMs may worsen voiding patterns.

Original languageEnglish (US)
Pages (from-to)1775-1781
Number of pages7
JournalBJU International
Volume106
Issue number11
DOIs
StatePublished - Dec 2010

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Postpartum Period
Histology
Growth Hormone
Elastin
Urethra
Adrenergic Receptors
Therapeutics
Pressure
Messenger RNA
Sprague Dawley Rats
Real-Time Polymerase Chain Reaction
Dilatation
Estrogens
Urinary Bladder
Immunohistochemistry
Parturition
Hormones
Control Groups
Raloxifene Hydrochloride
levormeloxifene

Keywords

  • animal model
  • growth hormone
  • incontinence
  • oestrogen
  • selective oestrogen receptor modulators

ASJC Scopus subject areas

  • Urology

Cite this

The effect of long-term hormonal treatment on voiding patterns during filling cystometry and on urethral histology in a postpartum, ovariectomized female rat. / Breyer, Benjamin N.; Wang, Guifang; Lin, Guiting; Shindel, Alan W.; Yang, Rong; Lin, Ching Shwun; Lue, Tom F.

In: BJU International, Vol. 106, No. 11, 12.2010, p. 1775-1781.

Research output: Contribution to journalArticle

Breyer, Benjamin N. ; Wang, Guifang ; Lin, Guiting ; Shindel, Alan W. ; Yang, Rong ; Lin, Ching Shwun ; Lue, Tom F. / The effect of long-term hormonal treatment on voiding patterns during filling cystometry and on urethral histology in a postpartum, ovariectomized female rat. In: BJU International. 2010 ; Vol. 106, No. 11. pp. 1775-1781.
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T1 - The effect of long-term hormonal treatment on voiding patterns during filling cystometry and on urethral histology in a postpartum, ovariectomized female rat

AU - Breyer, Benjamin N.

AU - Wang, Guifang

AU - Lin, Guiting

AU - Shindel, Alan W.

AU - Yang, Rong

AU - Lin, Ching Shwun

AU - Lue, Tom F.

PY - 2010/12

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N2 - OBJECTIVE To study whether long-term treatment with oestrogen (E 2), selective E2 receptor modulators (SERMs), or growth hormone (GH) can prevent the development of abnormal voiding patterns during filling cystometry (CMG) in a postpartum, ovariectomized (Ovx) female rat. MATERIALS AND METHODS Immediately after spontaneous delivery, 60 primiparous Sprague-Dawley rats were randomly divided into six equal groups. One group served as uninjured sham controls and five groups underwent intravaginal balloon dilatation. On day seven, previously dilated rats underwent bilateral Ovx and implantation of a subcutaneous hormone-delivery pump. The five treatment groups received normal saline (control), E2, raloxifene, levormeloxifene, or GH for 7 weeks. Conscious CMG was performed 7 weeks after Ovx. Urethral sphincter tissue was harvested for elastin immunohistochemistry and real-time polymerase chain reaction of α1A-adrenoceptor mRNA. RESULTS No abnormal voiding patterns were detected in the group treated with GH. The E 2, raloxifene and levormeloxifene groups had greater detrusor overactivity and urethral relaxation incontinence than control rats. The raloxifene group had a significantly lower baseline bladder pressure and opening pressure. GH-treated rats had higher elastin content in the urethra. Urethral α1A-adrenoceptor mRNA concentration was significantly lower in the SERM-treated rats compared with controls. CONCLUSIONS GH prevents the development of abnormal voiding patterns during filling CMG in a rat model of parturition-induced incontinence; E2 and SERMs may worsen voiding patterns.

AB - OBJECTIVE To study whether long-term treatment with oestrogen (E 2), selective E2 receptor modulators (SERMs), or growth hormone (GH) can prevent the development of abnormal voiding patterns during filling cystometry (CMG) in a postpartum, ovariectomized (Ovx) female rat. MATERIALS AND METHODS Immediately after spontaneous delivery, 60 primiparous Sprague-Dawley rats were randomly divided into six equal groups. One group served as uninjured sham controls and five groups underwent intravaginal balloon dilatation. On day seven, previously dilated rats underwent bilateral Ovx and implantation of a subcutaneous hormone-delivery pump. The five treatment groups received normal saline (control), E2, raloxifene, levormeloxifene, or GH for 7 weeks. Conscious CMG was performed 7 weeks after Ovx. Urethral sphincter tissue was harvested for elastin immunohistochemistry and real-time polymerase chain reaction of α1A-adrenoceptor mRNA. RESULTS No abnormal voiding patterns were detected in the group treated with GH. The E 2, raloxifene and levormeloxifene groups had greater detrusor overactivity and urethral relaxation incontinence than control rats. The raloxifene group had a significantly lower baseline bladder pressure and opening pressure. GH-treated rats had higher elastin content in the urethra. Urethral α1A-adrenoceptor mRNA concentration was significantly lower in the SERM-treated rats compared with controls. CONCLUSIONS GH prevents the development of abnormal voiding patterns during filling CMG in a rat model of parturition-induced incontinence; E2 and SERMs may worsen voiding patterns.

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KW - selective oestrogen receptor modulators

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