The effect of hypoxia on in vitro prevascularization of a thick soft tissue.

Craig K. Griffith, Steven George

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Prevascularizing an implantable tissue is one strategy to improve oxygen (O(2)) transport throughout larger tissues upon implantation. This study examined the role of hypoxia both during (i.e., as a stimulus) and after (i.e., mimicking implant conditions) vascularization of an implantable tissue. Tissues consisted of microcarrier beads coated with human umbilical vein endothelial cells embedded in fibrin. The fibrin was covered with a monolayer of normal human lung fibroblasts (NHLFs), or exposed to conditioned media from NHLFs. Capillary networks developed at 20% or 1% O(2) tension for 8 days. In some experiments, tissues were supplemented with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor, whereas in others the tissues prevascularized at 20% O(2) were transferred to 1% O(2) for 8 additional days. Maximal capillary formation occurred in media conditioned by NHLFs at 20% O(2), supplemented with VEGF (concentration >10 pM). Hypoxia (1% O(2)) did not stimulate basic fibroblast growth factor production and decreased in vitro angiogenesis, despite an increase in endogenous VEGF production. Hypoxia also degraded a preformed capillary network within 4 days. Hence, strategies to prevascularize implantable tissues may not require the physical presence of stromal cells, but will likely require fibroblast-derived growth factors in addition to VEGF to maintain capillary growth.

Original languageEnglish (US)
Pages (from-to)2423-2434
Number of pages12
JournalTissue engineering. Part A
Volume15
Issue number9
DOIs
StatePublished - Sep 1 2009

Fingerprint

Fibroblasts
Tissue
Vascular Endothelial Growth Factor A
Fibroblast Growth Factor 2
Conditioned Culture Medium
Fibrin
Lung
Fibroblast Growth Factors
Endothelial cells
Human Umbilical Vein Endothelial Cells
Stromal Cells
In Vitro Techniques
Hypoxia
Intercellular Signaling Peptides and Proteins
Monolayers
Oxygen
Growth
Experiments

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

The effect of hypoxia on in vitro prevascularization of a thick soft tissue. / Griffith, Craig K.; George, Steven.

In: Tissue engineering. Part A, Vol. 15, No. 9, 01.09.2009, p. 2423-2434.

Research output: Contribution to journalArticle

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