The effect of hepatic microsomal and cytosolic subcellular fractions on the mutagenic activity of epoxide-containing compounds in the salmonella assay

M. A. El-Tantawy, B. D. Hammock

Research output: Chapter in Book/Report/Conference proceedingChapter

41 Scopus citations

Abstract

7 epoxide-containing compounds: allylbenzene oxide, styrene oxide, trans-β-methylstyrene oxide, 4-chlorophenyl glycidyl ether, vinylcyclohexene dioxide, octene dioxide and hexene dioxide were evaluated for mutagenic activity in 4 histidine-requiring strains of Salmonella typhimurium, namely: TA1535, TA100, TA1537 and TA98. These epoxides, except trans-β-methylstyrene oxide, were mutagenic in TA1535 and TA100 but none of the tested compounds caused mutations in strains TA1537 and TA98. Both the cytosolic 100,000 g soluble) and/or microsomal 100,000 g pellet) fractions derived from non-induced mouse, guinea pig, and/or rat consistently decreased the mutagenic activity of the 3 most active mutagens: allylbenzene oxide, styrene oxide and 4-chlorophenyl glycidyl ether. This reduction was found to depend on the substrate and the source of the enzyme fraction. Glutathione alone or in combination with the mouse cytosolic fraction resulted in negligible suppression in the mutagenic activity of the 3 epoxides under the conditions reported in this paper. The enzyme(s) in the cytosol responsible for the reduction in mutagenicity co-eluted from gel filtration with the epoxide hydrolase activity. These data are not consistent with the assumption that all epoxide hydrolase activity in an 'S9' fraction is microsomal.

Original languageEnglish (US)
Title of host publicationMutation Research
Pages59-71
Number of pages13
Volume79
Edition1
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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